Aige-Gil V, Simkiss K
Department of Pure and Applied Zoology, School of Animal and Microbial Sciences, University of Reading.
Res Vet Sci. 1991 Mar;50(2):139-44. doi: 10.1016/0034-5288(91)90096-7.
The chemosterilant drug busulphan (1, 4-butanediol dimethane sulphonate) has been applied to embryos of the domestic fowl at doses of from 1 to 500 micrograms after 48 hours incubation. Direct application caused a number of teratological effects. These could be avoided by injecting the drug in sesame oil directly into the yolk. Embryos treated in this way could be sterilised with an efficiency of over 95 per cent with minimal side effects to the young embryo. The technique provides a method for destroying primordial germ cells that will facilitate studies of germ cell/stroma interactions in the gonad and facilitate the production of transgenic birds.
化学绝育药物白消安（1,4 - 丁二醇二甲磺酸酯）在孵化48小时后，已以1至500微克的剂量应用于家鸡胚胎。直接应用会产生一些致畸作用。通过将药物溶解在芝麻油中直接注入蛋黄，可以避免这些作用。以这种方式处理的胚胎能够以超过95%的效率进行绝育，对幼胚的副作用最小。该技术提供了一种破坏原始生殖细胞的方法，这将有助于研究性腺中生殖细胞/基质的相互作用，并有助于生产转基因鸟类。
