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核心技术专利：CN118964589B侵权必究

核心技术专利：CN118964589B侵权必究

Aige-Gil V, Simkiss K

Department of Pure and Applied Zoology, University of Reading, Whiteknights, England.

Br Poult Sci. 1991 Jul;32(3):427-38. doi: 10.1080/00071669108417368.

Experiments were undertaken to attempt to sterilise fowl embryos with ultraviolet light. Such sterilised embryos would be useful as recipients of genetically manipulated germ cells. 2. The germinal crescents of embryos were exposed to a calibrated UV source at stages 4 and 8 to 10 of incubation for 30 s, 3 min and 10 min. Teratological and sterility effects were studied at periods up to 6 d of incubation. 3. Simply exposing embryos by opening the shell produced a number of abnormalities and mortalities. These decreased with the age of the embryo but increased with the dosage of irradiation. 4. Although there was abundant evidence for UV-induced cell damage, the sterility of the embryos was usually less than 75%.

开展了用紫外线对鸡胚进行灭菌的实验。这种经灭菌的胚胎可作为基因操作生殖细胞的受体。2. 在孵化的第4阶段以及第8至10阶段，将胚胎的生殖新月暴露于校准的紫外线光源下30秒、3分钟和10分钟。在长达6天的孵化期内研究致畸和不育效应。3. 仅仅通过打开蛋壳暴露胚胎会产生一些异常和死亡情况。这些异常和死亡随着胚胎年龄的增长而减少，但随着辐照剂量的增加而增加。4. 尽管有大量证据表明紫外线会导致细胞损伤，但胚胎的不育率通常低于75%。

Aige-Gil V, Simkiss K

Department of Pure and Applied Zoology, University of Reading, Whiteknights, England.

Br Poult Sci. 1991 Jul;32(3):427-38. doi: 10.1080/00071669108417368.

Experiments were undertaken to attempt to sterilise fowl embryos with ultraviolet light. Such sterilised embryos would be useful as recipients of genetically manipulated germ cells. 2. The germinal crescents of embryos were exposed to a calibrated UV source at stages 4 and 8 to 10 of incubation for 30 s, 3 min and 10 min. Teratological and sterility effects were studied at periods up to 6 d of incubation. 3. Simply exposing embryos by opening the shell produced a number of abnormalities and mortalities. These decreased with the age of the embryo but increased with the dosage of irradiation. 4. Although there was abundant evidence for UV-induced cell damage, the sterility of the embryos was usually less than 75%.

开展了用紫外线对鸡胚进行灭菌的实验。这种经灭菌的胚胎可作为基因操作生殖细胞的受体。2. 在孵化的第4阶段以及第8至10阶段，将胚胎的生殖新月暴露于校准的紫外线光源下30秒、3分钟和10分钟。在长达6天的孵化期内研究致畸和不育效应。3. 仅仅通过打开蛋壳暴露胚胎会产生一些异常和死亡情况。这些异常和死亡随着胚胎年龄的增长而减少，但随着辐照剂量的增加而增加。4. 尽管有大量证据表明紫外线会导致细胞损伤，但胚胎的不育率通常低于75%。