Na(+):谷氨酸转运体同源物 Glt(Ph)中的天冬氨酸偶联计量比。
Na(+):aspartate coupling stoichiometry in the glutamate transporter homologue Glt(Ph).
机构信息
Department of Biochemistry, University of Groningen, Groningen Biomolecular Science and Biotechnology Institute, Nijenborgh 4, 9747 AG Groningen, The Netherlands.
出版信息
Biochemistry. 2010 May 4;49(17):3511-3. doi: 10.1021/bi100430s.
The Na(+) aspartate symporter Glt(Ph) from Pyrococcus horikoshii is the only member of the glutamate transporter family for which crystal structures have been determined. The cation:aspartate coupling stoichiometry is unknown, thus hampering the elucidation of the ion coupling mechanism. Here we measure transport of (22)Na(+) and [(14)C]aspartate in proteoliposomes containing purified Glt(Ph) and demonstrate that three Na(+) ions are symported with aspartate.
来自 Pyrococcus horikoshii 的 Na(+) 天冬氨酸协同转运蛋白 Glt(Ph) 是唯一一种已确定晶体结构的谷氨酸转运蛋白家族成员。阳离子：天冬氨酸偶联计量比未知，从而阻碍了离子偶联机制的阐明。在这里，我们在含有纯化的 Glt(Ph) 的脂质体中测量 (22)Na(+) 和 [(14)C]天冬氨酸的转运，并证明三个 Na(+) 离子与天冬氨酸协同转运。
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