Institute of Microanalytical Systems, Department of Chemistry, Zhejiang University, Hangzhou, 310058, China.
Anal Chem. 2010 Apr 15;82(8):3394-8. doi: 10.1021/ac100257z.
This paper reports a fully integrated hand-held photometer based on the liquid-core waveguide (LCW) detection principle for nanoliter-scale samples. All components of the photometer including light-emitting diode (LED) light source, LCW flow cell, photodiode detector, dropper pump, electronic circuit, liquid-crystal display screen, and battery were fully integrated into a small-sized (12 x 4.5 x 2.1 cm) instrument. A bent optical coupler was developed to conduct the detection light into or out of the LCW flow cell through its sidewall. This design allowed the sampling probe, input and output optical couplers, and LCW flow cell to be integrated in a single Teflon AF capillary, which significantly simplified system structure, improved working reliability, and reduced sample consumption. Two UV-LEDs were used as light source in the photometer to achieve dual wavelength detection at 260 and 280 nm, which was applied to assess on-site the quality and quantity of DNA samples. The effective optical path length of the photometer was approximately 15 mm with a sample consumption of only 350 nL. The potential of the photometer applied in point of care testing was also demonstrated in the measurement of total cholesterol in serum samples.
本文报道了一种基于液芯波导（LCW）检测原理的全集成手持式光度计，用于纳升级样品。光度计的所有组件，包括发光二极管（LED）光源、LCW 流池、光电二极管检测器、滴液泵、电子电路、液晶显示屏和电池，都被完全集成到一个小型（12 x 4.5 x 2.1 厘米）仪器中。开发了一个弯曲的光耦合器，通过其侧壁将检测光导入或导出 LCW 流池。这种设计允许采样探头、输入和输出光耦合器以及 LCW 流池集成在一个单一的聚四氟乙烯 AF 毛细管中，这大大简化了系统结构，提高了工作可靠性，并且减少了样品消耗。光度计中使用了两个 UV-LED 作为光源，实现了 260nm 和 280nm 的双波长检测，用于现场评估 DNA 样品的质量和数量。光度计的有效光程约为 15mm，样品消耗量仅为 350nL。光度计在现场检测中的应用潜力也在血清样品总胆固醇的测量中得到了证明。
