Department of Physiology, Faculty of Medicine, Firat University, Elazig, Turkey.
Biol Trace Elem Res. 2011 Mar;139(3):347-55. doi: 10.1007/s12011-010-8669-2. Epub 2010 Mar 30.
We investigated the effect of 17β-estradiol (E(2)) alone and separately vitamin E treatment on trace element status of rats following an ovariectomic operation. Forty rats were equally divided into four groups: Group 1, control, non-ovariectomized rats; Group 2, (OVX) rats, ovariectomized under general anesthesia; Group 3, (OVX+E(2)) rats, the group received a 40 µg kg(-1) subcutan dose of E(2) per day after ovariectomy; and Group 4, (OVX + E(2) + vitamin E) rats, received the same E(2) treatment, but with an additional 100 mg kg(-1) intraperitoneal dose of vitamin E per day after ovariectomy. At the end of the 30-day experiment, the rats were sacrificed and their blood was collected for the measurement of zinc, copper, iron, phosphorus, selenium, magnesium, calcium, manganese, and chromium; copper-zinc superoxide dismutase (SOD); manganese-superoxide dismutase (Mn-SOD); glutathione peroxidase (Se-GSH-Px); and catalase (CAT). The levels of zinc, copper, iron, phosphorus, selenium, calcium, chromium, and manganese and activities of SOD, Mn-SOD, Se-GSH-Px, and CAT were lower in the OVX than in the control group, but magnesium level was unaffected. However, zinc, copper, iron, phosphorus, selenium, calcium, chromium, and manganese levels and SOD, Mn-SOD, Se-GSH-Px, and CAT activities were higher under separate E(2) and E(2) + vitamin E treatments. The level of magnesium in the treated-OVX groups was not different than in the OVX group. In conclusion, E(2) treatment has an ameliorating effect on the trace element status in OVX, and this effect may be enhanced with the addition of vitamin E.
我们研究了 17β-雌二醇（E（2））单独和分别用维生素 E 处理对去卵巢手术后大鼠微量元素状态的影响。40 只大鼠等分为 4 组：第 1 组，对照组，未去卵巢大鼠；第 2 组，（OVX）大鼠，在全身麻醉下进行去卵巢手术；第 3 组，（OVX+E（2））大鼠，每天接受 40µgkg（-1）皮下剂量的 E（2）；第 4 组，（OVX+E（2）+维生素 E）大鼠，接受相同的 E（2）治疗，但在去卵巢手术后每天额外接受 100mgkg（-1）腹腔剂量的维生素 E。在 30 天实验结束时，处死大鼠并采集血液，用于测量锌、铜、铁、磷、硒、镁、钙、锰和铬；铜锌超氧化物歧化酶（SOD）；锰超氧化物歧化酶（Mn-SOD）；谷胱甘肽过氧化物酶（Se-GSH-Px）；和过氧化氢酶（CAT）。与对照组相比，OVX 组的锌、铜、铁、磷、硒、钙、铬和锰水平以及 SOD、Mn-SOD、Se-GSH-Px 和 CAT 活性较低，但镁水平不受影响。然而，单独的 E（2）和 E（2）+维生素 E 处理后，锌、铜、铁、磷、硒、钙、铬和锰水平以及 SOD、Mn-SOD、Se-GSH-Px 和 CAT 活性较高。治疗 OVX 组的镁水平与 OVX 组无差异。总之，E（2）治疗对 OVX 大鼠微量元素状态有改善作用，这种作用可能因添加维生素 E 而增强。
