Sun Xiaoying, Li Ying, Liu Li, Zhang Chen, Li Bin, Liang Tongwen
Shenzhen Entry-Exit Inspection and Quarantine Bureau, Shenzhen 518045, China.
Se Pu. 2009 Nov;27(6):852-5.
An analytical method based on high performance liquid chromatography with diode array detector (HPLC-DAD) has been established for the simultaneous determination of 9 water-soluble colorants (Solvent Green 7, Food Yellow 3, Food Red 17, Acid Yellow 1, Acid Red 33, Food Red 4, Food Red 1, Orange I, Acid Orange 7) in cosmetics. The different kinds of samples were treated with different preparation methods. The obtained liquid samples were analyzed by HPLC using a Diamonsil C18 column (250 mm x 4.6 mm, 5 microm). Acetonitrile-potassium dihydrogen phosphate (KH2PO4) buffer solution (pH 6) was used as the mobile phase for gradient elution. The 9 water-soluble colorants were well separated within 15 min. The average recoveries (n = 9) were from 85.33% to 100.2% with the relative standard deviations (RSDs) between 3.68% and 8.20%. The limits of detection (LOD) were in the range of 0.01-0.1 mg/L. The method is simple, rapid, accurate and suitable for the determination of 9 water-soluble colorants in cosmetics.
建立了一种基于高效液相色谱-二极管阵列检测器（HPLC-DAD）的分析方法，用于同时测定化妆品中的9种水溶性色素（溶剂绿7、食用黄3、食用红17、酸性黄1、酸性红33、食用红4、食用红1、橙黄Ⅰ、酸性橙7）。不同种类的样品采用不同的制备方法进行处理。所得液体样品采用Diamonsil C18柱（250 mm×4.6 mm，5μm）通过HPLC进行分析。以乙腈-磷酸二氢钾（KH2PO4）缓冲溶液（pH 6）作为流动相进行梯度洗脱。9种水溶性色素在15分钟内得到良好分离。平均回收率（n = 9）为85.33%至100.2%，相对标准偏差（RSD）在3.68%至8.20%之间。检测限（LOD）在0.01 - 0.1 mg/L范围内。该方法简便、快速、准确，适用于化妆品中9种水溶性色素含量的测定。
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