通过水、高氯酸和苯酚水溶液从粗球孢子菌菌丝体中提取皮肤试验活性物质。
Extraction of skin test activity from Coccidioides immitis mycelia by water, perchloric acid, and aqueous phenol extraction.
作者信息
Wheat R W, Su Chung K S, Ornellas E P, Scheer E R
出版信息
Infect Immun. 1978 Jan;19(1):152-9. doi: 10.1128/iai.19.1.152-159.1978.
Abstract
Water, perchloric acid extracts, and fractions of partially defatted whole mycelia of Coccidioides immitis contained delayed skin test activity when tested in C. immitis-infected guinea pits. Aqueous phenol extraction of these fractions resulted in partitioning of activity between aqueous-soluble and phenol-soluble fractions; activity was found to be water soluble after removal of phenol by extensive dialysis. Highest specific activity skin test antigen was invariably found in the phenol-soluble phase, water-soluble fraction. Material of equivalent activity could also be extracted directly from the defatted mycelia. Skin test active fractions contained glucose, mannose, 3-O-methylmannose, glucosamine, and amino acids.
摘要
当在感染球孢子菌的豚鼠身上进行测试时，粗球孢子菌的水、高氯酸提取物以及部分脱脂全菌丝体的组分具有迟发型皮肤试验活性。对这些组分进行水相苯酚萃取后，活性在水溶性和苯酚溶性组分之间进行了分配；通过广泛透析去除苯酚后，发现活性是水溶性的。最高比活性的皮肤试验抗原始终存在于苯酚溶性相的水溶性组分中。具有同等活性的物质也可以直接从脱脂菌丝体中提取。皮肤试验活性组分含有葡萄糖、甘露糖、3 - O - 甲基甘露糖、氨基葡萄糖和氨基酸。
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