ACS Appl Mater Interfaces. 2009 Feb;1(2):232-4. doi: 10.1021/am800093d.
For the first time, a conjugated fluorescent polymer was utilized to probe alpha-amino acids, sensitively and selectively, through a new approach. First the strong fluorescence of the prepared polyfluorene (P1) was quenched by trace copper ions, and then the quenched fluorescence was recovered upon the addition of alpha-amino acids, making P1 a new and sensitive biosensor toward alpha-amino acids. The experimental results demonstrated that the alpha-amino acid selective nature of P1 over other analytes was relatively good. Thus, the work reported here might open up a new avenue for developing new biosensors.
首次利用共轭荧光聚合物通过一种新方法灵敏且选择性地探测α-氨基酸。首先，通过微量铜离子猝灭所制备的聚芴（P1）的强荧光，然后通过加入α-氨基酸恢复猝灭的荧光，使得 P1 成为一种新的、灵敏的α-氨基酸生物传感器。实验结果表明，P1 对其他分析物具有相对较好的α-氨基酸选择性。因此，这里报道的工作可能为开发新型生物传感器开辟新途径。
