Institute of Medicine, University of Bergen, Norway.
Aliment Pharmacol Ther. 2010 Jul;32(1):66-73. doi: 10.1111/j.1365-2036.2010.04314.x. Epub 2010 Mar 26.
Medically confirmed hypersensitivity reactions to food are usually IgE-mediated. Non-IgE-mediated reactions are not only seldom recognized but also more difficult to diagnose.
To examine B cell-activating factor (BAFF) in serum and gut lavage fluid of patients with self-reported food hypersensitivity, and to study its relationship to atopic disease.
Gut lavage fluid was obtained from 60 and serum from another 17 patients with self-reported food hypersensitivity. Twenty healthy volunteers served as controls, gut lavage fluid was obtained in all, serum from 11 of 20. The patients were divided into atopic and non-atopic subgroups. BAFF was measured by ELISA in both serum and gut lavage fluid.
B cell-activating factor levels in serum and gut lavage fluid were significantly higher in patients than in controls (P < 0.03 and P < 0.002 respectively). Non-atopic patients had significantly higher levels of BAFF in serum than both atopic patients (P < 0.05) and controls (P < 0.05). There was no significant correlation between serum levels of BAFF and IgE.
The results suggest that BAFF might be a new mediating mechanism in food hypersensitivity reactions. Significantly higher levels in non-atopic compared with atopic patients, and no correlation between BAFF and IgE, suggest that BAFF might be involved particularly in non-IgE-mediated reactions.
经医学确认的食物过敏反应通常为 IgE 介导。非 IgE 介导的反应不仅很少被识别，而且更难诊断。
检测自身报告食物过敏患者血清和肠灌洗液中的 B 细胞激活因子（BAFF），并研究其与特应性疾病的关系。
从 60 例自述食物过敏的患者中获取肠灌洗液，从另外 17 例患者中获取血清。20 名健康志愿者作为对照，所有对照均获取肠灌洗液，其中 11 名对照获取血清。将患者分为特应性和非特应性亚组。通过 ELISA 法在血清和肠灌洗液中测量 BAFF。
患者的血清和肠灌洗液中的 BAFF 水平明显高于对照组（P<0.03 和 P<0.002）。非特应性患者的血清 BAFF 水平明显高于特应性患者（P<0.05）和对照组（P<0.05）。血清 BAFF 水平与 IgE 之间无显著相关性。
结果表明 BAFF 可能是食物过敏反应的新介导机制。非特应性患者明显高于特应性患者，且 BAFF 与 IgE 之间无相关性，提示 BAFF 可能特别参与非 IgE 介导的反应。
