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应用低温胁迫对秀丽隐杆线虫进行年龄分离：一种简单廉价的技术。

Applications of cold temperature stress to age fractionate Caenorhabditis elegans: a simple inexpensive technique.

机构信息

Department of Molecular and Microbiology, George Mason University, 161 Discovery Hall, PW, MSN: 4E3, Manassas, VA 20110, USA.

出版信息

J Gerontol A Biol Sci Med Sci. 2010 May;65(5):457-67. doi: 10.1093/gerona/glq036. Epub 2010 Mar 30.

DOI:10.1093/gerona/glq036
PMID:20354064
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2854889/
Abstract

The nematode Caenorhabditis elegans's (CE) successful use in studies of aging is well documented. Cold temperature stress of mixed populations of CE provides a rapid inexpensive means of obtaining three life stage-specific cohorts. Cohorts are obtained in quantities that allow acquisition of replicate metabolite profiles of changes associated with development, aging, and senescence. The fractionation technique is effective with monoxenic and axenic CE cultures. Cohort Y contains 100% young worms, and Cohort A contains 75% adult worms. Cohort M, prereproductive and reproductive, contains some A and Y due to continuous egg laying and hatch. Principal component analysis of normalized data from metabolite profiles obtained using high-performance liquid chromatography electrochemical analysis clearly separates Cohort Y from Cohort A and monoxenic from axenic cultured worms. Access to replicate quantities of age-defined worms will aid studies of alterations in homeostatic controls associated with aging and senescence.

摘要

秀丽隐杆线虫（CE）在衰老研究中的成功应用已有充分记录。混合种群 CE 的低温应激提供了一种快速、廉价的方法来获得三个特定生命阶段的群体。获得的群体数量允许获得与发育、衰老和衰老相关的代谢物变化的重复特征图谱。该分馏技术对单养和混养 CE 培养物均有效。群体 Y 包含 100%的幼体蠕虫，群体 A 包含 75%的成年蠕虫。群体 M 是预生殖和生殖的，由于持续产卵和孵化，因此含有一些 A 和 Y。使用高效液相色谱电化学分析获得的代谢物特征图谱的归一化数据的主成分分析清楚地区分了群体 Y 与群体 A 以及单养和混养培养的蠕虫。获得大量具有年龄定义的蠕虫将有助于研究与衰老和衰老相关的体内平衡控制的变化。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/208a/2854889/af31f33de515/geronaglq036f05_3c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/208a/2854889/2ec483c66b7e/geronaglq036f01_ht.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/208a/2854889/3102de588158/geronaglq036f02_3c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/208a/2854889/e502ca5013db/geronaglq036f03_ht.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/208a/2854889/64624fbbef69/geronaglq036f04_ht.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/208a/2854889/af31f33de515/geronaglq036f05_3c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/208a/2854889/2ec483c66b7e/geronaglq036f01_ht.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/208a/2854889/3102de588158/geronaglq036f02_3c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/208a/2854889/e502ca5013db/geronaglq036f03_ht.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/208a/2854889/64624fbbef69/geronaglq036f04_ht.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/208a/2854889/af31f33de515/geronaglq036f05_3c.jpg

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