• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

卵巢癌干细胞样侧群细胞具有致瘤性和化疗耐药性。

Ovarian cancer stem-like side-population cells are tumourigenic and chemoresistant.

机构信息

Center for Reproductive Sciences, Department of Obstetrics, Gynecology and Reproductive Sciences, University of California, San Francisco, 94143-0556, USA.

出版信息

Br J Cancer. 2010 Apr 13;102(8):1276-83. doi: 10.1038/sj.bjc.6605626. Epub 2010 Mar 30.

DOI:10.1038/sj.bjc.6605626
PMID:20354527
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2856005/
Abstract

BACKGROUND

Ovarian cancer is the most lethal gynaecological malignancy. Although ovarian cancer patients often respond initially to chemotherapy, they usually develop chemoresistance. We hypothesised that a small portion of ovarian cancer cells have stem-like cell properties that contribute to tumourigenesis and drug resistance.

METHODS

Flow cytometry and Hoechst 33342 efflux isolated side-population (SP) cells from ascites derived from ovarian cancer patients and from mice inoculated with human ovarian cancer cell lines. The SP cells were examined for stem cell markers OCT4, NANOG, STELLAR, and ABCG2/BCRP1 by immunocytochemistry and RT-PCR. The SP cells and non-SP cells were studied for tumourigenesis and chemoresistance in vitro and in vivo.

RESULTS

The SP cells expressed ABCG2/BCRP1, OCT4, STELLAR, and NANOG, detected by immunocytochemistry and RT-PCR. ABCG2/BCRP1 expression was higher in SP than in non-SP cells. Xenogeneic mice inoculated with SP cells yielded more tumours than did mice inoculated with non-SP cells. In parallel, SP cell culture resulted in extensive cell proliferation, which was markedly more than in non-SP cells. SP cells resisted chemotherapy compared with non-SP cells, both in vivo and in vitro.

CONCLUSION

Ovarian cancer SP cells are tumourigenic and chemoresistant. ABCG2/BCRP1 has an important role in chemoresistance, which has implications for new therapeutic approaches.

摘要

背景

卵巢癌是最致命的妇科恶性肿瘤。尽管卵巢癌患者最初通常对化疗有反应，但他们通常会产生耐药性。我们假设一小部分卵巢癌细胞具有促进肿瘤发生和耐药的干细胞样细胞特性。

方法

利用流式细胞术和 Hoechst 33342 外排分离来自卵巢癌患者腹水和接种人卵巢癌细胞系的小鼠的侧群（SP）细胞。通过免疫细胞化学和 RT-PCR 检查 SP 细胞中的干细胞标志物 OCT4、NANOG、STELLAR 和 ABCG2/BCRP1。研究 SP 细胞和非 SP 细胞在体外和体内的致瘤性和化疗耐药性。

结果

SP 细胞通过免疫细胞化学和 RT-PCR 表达 ABCG2/BCRP1、OCT4、STELLAR 和 NANOG。SP 细胞中的 ABCG2/BCRP1 表达高于非 SP 细胞。用 SP 细胞接种的异种小鼠比用非 SP 细胞接种的小鼠产生更多的肿瘤。平行地，SP 细胞培养导致广泛的细胞增殖，明显多于非 SP 细胞。SP 细胞对化疗的抵抗力比非 SP 细胞更强，无论是在体内还是在体外。

结论

卵巢癌 SP 细胞具有致瘤性和化疗耐药性。ABCG2/BCRP1 在化疗耐药中起重要作用，这对新的治疗方法具有重要意义。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9cd3/2856005/a486ac452c5d/6605626f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9cd3/2856005/70ff7e3f263a/6605626f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9cd3/2856005/2cdaae4e2541/6605626f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9cd3/2856005/b2771bd2ee12/6605626f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9cd3/2856005/7ee1994ab7da/6605626f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9cd3/2856005/a486ac452c5d/6605626f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9cd3/2856005/70ff7e3f263a/6605626f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9cd3/2856005/2cdaae4e2541/6605626f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9cd3/2856005/b2771bd2ee12/6605626f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9cd3/2856005/7ee1994ab7da/6605626f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9cd3/2856005/a486ac452c5d/6605626f5.jpg

相似文献

1
Ovarian cancer stem-like side-population cells are tumourigenic and chemoresistant.卵巢癌干细胞样侧群细胞具有致瘤性和化疗耐药性。
Br J Cancer. 2010 Apr 13;102(8):1276-83. doi: 10.1038/sj.bjc.6605626. Epub 2010 Mar 30.
2
[Analysis of the characteristics of side population cells in the human ovarian cancer cell line OVCAR-3].[人卵巢癌细胞系OVCAR-3中侧群细胞特性分析]
Zhonghua Fu Chan Ke Za Zhi. 2012 Apr;47(4):281-5.
3
Using ABCG2-molecule-expressing side population cells to identify cancer stem-like cells in a human ovarian cell line.利用 ABCG2 分子表达的侧群细胞鉴定人卵巢癌细胞系中的癌症干细胞样细胞。
Cell Biol Int. 2011 Mar;35(3):227-34. doi: 10.1042/CBI20100347.
4
The multidrug resistance transporter ABCG2 (breast cancer resistance protein 1) effluxes Hoechst 33342 and is overexpressed in hematopoietic stem cells.多药耐药转运蛋白ABCG2（乳腺癌耐药蛋白1）可外排Hoechst 33342，且在造血干细胞中过表达。
Clin Cancer Res. 2002 Jan;8(1):22-8.
5
Emodin as an effective agent in targeting cancer stem-like side population cells of gallbladder carcinoma.大黄素作为一种有效药物靶向胆囊癌细胞癌干细胞样侧群细胞。
Stem Cells Dev. 2013 Feb 15;22(4):554-66. doi: 10.1089/scd.2011.0709. Epub 2012 Nov 7.
6
Tumor recurrence and drug resistance properties of side population cells in high grade ovary cancer.高级别卵巢癌中侧群细胞的肿瘤复发及耐药特性
Drug Res (Stuttg). 2015 Mar;65(3):153-7. doi: 10.1055/s-0034-1375609. Epub 2014 Dec 11.
7
Biologic characteristics of the side population of human small cell lung cancer cell line H446.人小细胞肺癌细胞系H446侧群细胞的生物学特性
Chin J Cancer. 2010 Mar;29(3):254-60. doi: 10.5732/cjc.009.10330.
8
Low molecular weight heparin ablates lung cancer cisplatin-resistance by inducing proteasome-mediated ABCG2 protein degradation.低分子量肝素通过诱导蛋白酶体介导的 ABCG2 蛋白降解来消除肺癌顺铂耐药性。
PLoS One. 2012;7(7):e41035. doi: 10.1371/journal.pone.0041035. Epub 2012 Jul 23.
9
Side population cells isolated from KATO III human gastric cancer cell line have cancer stem cell-like characteristics.从 KATO III 人胃癌细胞系中分离出的侧群细胞具有癌症干细胞样特征。
World J Gastroenterol. 2012 Sep 7;18(33):4610-7. doi: 10.3748/wjg.v18.i33.4610.
10
Side population in human non-muscle invasive bladder cancer enriches for cancer stem cells that are maintained by MAPK signalling.人非肌肉浸润性膀胱癌的侧群细胞中富含肿瘤干细胞，这些干细胞由 MAPK 信号维持。
PLoS One. 2012;7(11):e50690. doi: 10.1371/journal.pone.0050690. Epub 2012 Nov 30.

引用本文的文献

1
Role of in regulation of Paclitaxel response and EMT process during ovarian tumor progression.[此处原文不完整，推测可能是某个因素的名称未给出，暂无法准确翻译完整句子]在卵巢肿瘤进展过程中对紫杉醇反应和上皮-间质转化过程的调节作用。
Mol Biol Res Commun. 2025;14(4):249-258. doi: 10.22099/mbrc.2025.52515.2107.
2
AAV for ovarian cancer gene therapy.用于卵巢癌基因治疗的腺相关病毒
Cancer Gene Ther. 2025 Jun 20. doi: 10.1038/s41417-025-00926-4.
3
Targeted Radionuclide Therapy Using a Lutetium-177 Labeled Human Anti-CD133 Antibody.使用镥-177标记的人抗CD133抗体的靶向放射性核素治疗

本文引用的文献

1
Cancer stem cells and their niche.癌症干细胞及其微环境。
Cancer Sci. 2009 Jul;100(7):1166-72. doi: 10.1111/j.1349-7006.2009.01177.x. Epub 2009 Apr 22.
2
Cancer stem cells are enriched in the side population cells in a mouse model of glioma.在胶质瘤小鼠模型中，癌症干细胞在侧群细胞中富集。
Cancer Res. 2008 Dec 15;68(24):10051-9. doi: 10.1158/0008-5472.CAN-08-0786.
3
The polycomb gene product BMI1 contributes to the maintenance of tumor-initiating side population cells in hepatocellular carcinoma.多梳基因产物BMI1有助于维持肝细胞癌中具有肿瘤起始能力的侧群细胞。
Mol Imaging Biol. 2025 May 22. doi: 10.1007/s11307-025-02013-4.
4
Co-expression of HIF1A with multi-drug transporters (P-GP, MRP1, and BCRP) in chemoresistant breast, colorectal, and ovarian cancer cells.缺氧诱导因子1α（HIF1A）与多药转运蛋白（P-糖蛋白、多药耐药相关蛋白1和乳腺癌耐药蛋白）在耐化疗的乳腺癌、结直肠癌和卵巢癌细胞中的共表达。
J Genet Eng Biotechnol. 2025 Jun;23(2):100496. doi: 10.1016/j.jgeb.2025.100496. Epub 2025 May 1.
5
Role of KLF5 in enhancing ovarian cancer stemness and PARPi resistance: mechanisms and therapeutic targeting.KLF5在增强卵巢癌干性和PARP抑制剂耐药性中的作用：机制与治疗靶点
J Transl Med. 2025 Apr 30;23(1):492. doi: 10.1186/s12967-025-06502-6.
6
NCAPH promotes glucose metabolism reprogramming and cell stemness in ovarian cancer cells through the MEK/ERK/PD-L1 pathway.NCAPH通过MEK/ERK/PD-L1途径促进卵巢癌细胞中的葡萄糖代谢重编程和细胞干性。
J Ovarian Res. 2025 Apr 21;18(1):81. doi: 10.1186/s13048-025-01659-6.
7
Nanotechnology-based drug delivery system for the diagnosis and treatment of ovarian cancer.用于卵巢癌诊断与治疗的基于纳米技术的药物递送系统。
Discov Oncol. 2025 Mar 29;16(1):422. doi: 10.1007/s12672-025-02062-9.
8
A Peptide Derived from Nectin-4 Increases Cisplatin Cytotoxicity in Cell Lines and Cells from Ovarian Cancer Patients' Ascites.一种源自NECTIN-4的肽可增强顺铂对卵巢癌细胞系及卵巢癌患者腹水细胞的细胞毒性。
Cancers (Basel). 2025 Mar 6;17(5):901. doi: 10.3390/cancers17050901.
9
Role of Cancer Side Population Stem Cells in Ovarian Cancer Angiogenesis.癌症侧群干细胞在卵巢癌血管生成中的作用。
Med Princ Pract. 2024;33(5):403-413. doi: 10.1159/000539642. Epub 2024 Jul 26.
10
ARID1A restrains EMT and stemness of ovarian cancer cells through the Hippo pathway.ARID1A 通过 Hippo 通路抑制卵巢癌细胞的 EMT 和干性。
Int J Oncol. 2024 Aug;65(2). doi: 10.3892/ijo.2024.5664. Epub 2024 Jun 14.
Cancer Res. 2008 Oct 1;68(19):7742-9. doi: 10.1158/0008-5472.CAN-07-5882.
4
Quantitative mass spectrometry identifies drug targets in cancer stem cell-containing side population.定量质谱法鉴定含癌干细胞的侧群中的药物靶点。
Stem Cells. 2008 Dec;26(12):3037-46. doi: 10.1634/stemcells.2008-0397. Epub 2008 Sep 18.
5
An embryonic stem cell-like gene expression signature in poorly differentiated aggressive human tumors.低分化侵袭性人类肿瘤中的胚胎干细胞样基因表达特征
Nat Genet. 2008 May;40(5):499-507. doi: 10.1038/ng.127.
6
Module map of stem cell genes guides creation of epithelial cancer stem cells.干细胞基因模块图谱指导上皮癌干细胞的生成。
Cell Stem Cell. 2008 Apr 10;2(4):333-44. doi: 10.1016/j.stem.2008.02.009.
7
Stem cells and cancer: an overview.干细胞与癌症：概述
Stem Cell Rev. 2007 Dec;3(4):249-55. doi: 10.1007/s12015-007-9002-0.
8
Side population cells isolated from mesenchymal neoplasms have tumor initiating potential.从间充质肿瘤中分离出的侧群细胞具有肿瘤起始潜能。
Cancer Res. 2007 Sep 1;67(17):8216-22. doi: 10.1158/0008-5472.CAN-07-0999.
9
Side population in human lung cancer cell lines and tumors is enriched with stem-like cancer cells.人肺癌细胞系和肿瘤中的侧群富含干细胞样癌细胞。
Cancer Res. 2007 May 15;67(10):4827-33. doi: 10.1158/0008-5472.CAN-06-3557.
10
Identification of cancer stem cell-like side population cells in human nasopharyngeal carcinoma cell line.人鼻咽癌细胞系中癌干细胞样侧群细胞的鉴定
Cancer Res. 2007 Apr 15;67(8):3716-24. doi: 10.1158/0008-5472.CAN-06-4343.