Department of General Surgery, First Affiliated Hospital of Medical College, Xi'an Jiaotong University, Xi'an 710061, Shaanxi Province, China.
World J Gastroenterol. 2012 Sep 7;18(33):4610-7. doi: 10.3748/wjg.v18.i33.4610.
To investigate whether the side population (SP) cells possess cancer stem cell-like characteristics in vitro and the role of SP cells in tumorigenic process in gastric cancer.
We analyzed the presence of SP cells in different human gastric carcinoma cell lines, and then isolated and identified the SP cells from the KATO III human gastric cancer cell line by flow cytometry. The clonogenic ability and self-renewal were evaluated by clone and sphere formation assays. The related genes were determined by reverse transcription polymerase chain reaction. To compare tumorigenic ability, SP and non-side population (NSP) cells from the KATO III human gastric cancer cell line were subcutaneously injected into nude mice.
SP cells from the total population accounted for 0.57% in KATO III, 1.04% in Hs-746T, and 0.02% in AGS (CRL-1739). SP cells could grow clonally and have self-renewal capability in conditioned media. The expression of ABCG2, MDRI, Bmi-1 and Oct-4 was different between SP and NSP cells. However, there was no apparent difference between SP and NSP cells when they were injected into nude mice.
SP cells have some cancer stem cell-like characteristics in vitro and can be used for studying the tumorigenic process in gastric cancer.
研究胃癌细胞群体中是否存在具有肿瘤干细胞样特征的侧群（SP）细胞，以及 SP 细胞在胃癌发生过程中的作用。
我们分析了不同人胃癌细胞系中 SP 细胞的存在情况，然后通过流式细胞术从 KATO III 人胃癌细胞系中分离和鉴定 SP 细胞。通过克隆和球体形成实验评估其克隆形成能力和自我更新能力。通过反转录聚合酶链反应确定相关基因。为了比较致瘤能力，将 KATO III 人胃癌细胞系中的 SP 和非侧群（NSP）细胞皮下注射到裸鼠中。
KATO III 中的总群体中 SP 细胞占 0.57%，Hs-746T 中占 1.04%，AGS（CRL-1739）中占 0.02%。SP 细胞在条件培养基中能够进行克隆生长和自我更新。ABCG2、MDRI、Bmi-1 和 Oct-4 的表达在 SP 和 NSP 细胞之间存在差异。然而，当将 SP 和 NSP 细胞注射到裸鼠中时，它们之间没有明显的差异。
SP 细胞在体外具有一些肿瘤干细胞样特征，可用于研究胃癌的致瘤过程。
