Key Laboratory of Genome Information and Sciences, Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing, 100029, People's Republic of China.
Mar Biotechnol (NY). 2011 Apr;13(2):151-62. doi: 10.1007/s10126-010-9275-1. Epub 2010 Mar 31.
To identify muscle-related protein isoforms expressed in the white muscle of the mandarin fish Siniperca chuatsi, we analyzed 5,063 high-quality expressed sequence tags (ESTs) from white muscle cDNA library and predicted the integrity of the clusters annotated to these genes and the physiochemical properties of the putative polypeptides with full length. Up to about 33% of total ESTs were annotated to muscle-related proteins: myosin, actin, tropomyosin/troponin complex, parvalbumin, and Sarcoplasmic/endoplasmic reticulum calcium ATPase (SERCa). Thirty-two isoforms were identified and more than one isoform existed in each of these proteins. Among these isoforms, 14 putative polypeptides were with full length. In addition, about 2% of total ESTs were significantly homologous to "glue" molecules such as alpha-actinins, myosin-binding proteins, myomesin, tropomodulin, cofilin, profilin, twinfilins, coronin-1, and nebulin, which were required for the integrity and maintenance of the muscle sarcomere. The results demonstrated that multiple isoforms of major muscle-related proteins were expressed in S. chuatsi white muscle. The analysis on these isoforms and other proteins sequences will greatly aid our systematic understanding of the high flexibility of mandarin fish white muscle at molecular level and expand the utility of fish systems as models for the muscle genetic control and function.
为了鉴定白肌中表达的与肌肉相关的蛋白质同工型，我们分析了来自白肌 cDNA 文库的 5063 个高质量表达序列标签（EST），并预测了这些基因的聚类的完整性以及全长推定多肽的物理化学特性。约 33%的 EST 被注释为与肌肉相关的蛋白质：肌球蛋白、肌动蛋白、原肌球蛋白/肌钙蛋白复合物、副肌球蛋白和肌浆/内质网钙 ATP 酶（SERCa）。鉴定出 32 个同工型，这些蛋白质中的每个都存在不止一种同工型。在这些同工型中，有 14 个推定多肽具有全长。此外，约 2%的 EST 与“粘性”分子（如α肌动蛋白、肌球蛋白结合蛋白、肌联蛋白、原肌球蛋白、丝切蛋白、肌动蛋白丝结合蛋白、微管蛋白、原肌球蛋白、双filin 和 coronin-1）具有显著同源性，这些分子对于肌节的完整性和维持是必需的。结果表明，多种主要与肌肉相关的蛋白质同工型在白肌中表达。对这些同工型和其他蛋白质序列的分析将极大地帮助我们系统地理解白肌的高灵活性在分子水平上，并扩展鱼类系统作为肌肉遗传控制和功能模型的应用。
