The Key Laboratory for Microbial Resources of Ministry of Education, Yunnan Institute of Microbiology, Yunnan University, Kunming 650091, Yunnan, People's Republic of China.
Antonie Van Leeuwenhoek. 2010 Jun;98(1):77-83. doi: 10.1007/s10482-010-9431-7. Epub 2010 Mar 31.
During the course of our research on new actinobacterial sources, a novel actinomycete strain YIM 67013(T) was isolated from rhizosphere soil of Tripterygium wilfordii Hook. f. collected from Yunnan province, south-west China. The strain formed well-differentiated aerial and substrate mycelia, grew in the presence of up to 15% NaCl (optimum, 0-3% NaCl). Phylogenetic analysis based on 16S rRNA gene sequences showed that strain YIM 67013(T) belongs to the genus Pseudonocardia, with highest similarity to Pseudonocardia thermophila DSM 43832(T) (97.6%). Sequence similarities between strain YIM 67013(T) and the other Pseudonocardia species ranged from 96.6 to 93.2%. Key morphological and physiological characteristics as well as chemotaxonomic features of strain YIM 67013(T) were congruent with the description of the genus Pseudonocardia. The G+C content of the genomic DNA was 69.7 mol%. Based on comparative analysis of physiological, biochemical and chemotaxonomic data, including low DNA-DNA hybridization results, it is proposed that strain YIM 67013(T) represents a novel species of the genus Pseudonocardia, named Pseudonocardia rhizophila sp. nov. The type strain is YIM 67013(T) (= CCTCC AA 209043(T) = DSM 45381(T)).
在我们对新放线菌资源的研究过程中，从中国西南部云南省采集的三叶鬼臼根际土壤中分离到一株新型放线菌菌株 YIM 67013(T)。该菌株形成明显分化的气生和基生菌丝，在高达 15%的 NaCl 存在下生长（最佳，0-3%NaCl）。基于 16S rRNA 基因序列的系统发育分析表明，菌株 YIM 67013(T)属于假诺卡氏菌属，与嗜热假诺卡氏菌 DSM 43832(T)（97.6%）最为相似。菌株 YIM 67013(T)与其他假诺卡氏菌属的种之间的序列相似度在 96.6%至 93.2%之间。菌株 YIM 67013(T)的关键形态和生理特征以及化学分类学特征与假诺卡氏菌属的描述相符。基因组 DNA 的 G+C 含量为 69.7 mol%。基于生理、生化和化学分类学数据的比较分析，包括低 DNA-DNA 杂交结果，建议菌株 YIM 67013(T)代表假诺卡氏菌属的一个新种，命名为假诺卡氏菌根际亚种。模式菌株为 YIM 67013(T)（=CCTCC AA 209043(T)=DSM 45381(T)）。
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