The Key Laboratory for Microbial Resources of the Ministry of Education, Kunming, People's Republic of China.
Antonie Van Leeuwenhoek. 2010 Jun;98(1):31-8. doi: 10.1007/s10482-010-9425-5. Epub 2010 Mar 17.
A Gram-positive, moderately halophilic, facultatively alkaliphilic, catalase- and oxidase-positive, obligately aerobic, filamentous actinomycete strain, designated YIM 90022(T), was isolated from saline soil collected from the Qaidam Basin, north-west China. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the new isolate was a member of the genus Nocardiopsis and the sequence similarities between the isolate and the type strains of members of the genus Nocardiopsis were in the range of 95.1-98.7%. Phenotypic and chemotaxonomic properties of this organism also indicated that strain YIM 90022(T) was a member of the genus Nocardiopsis. The strain grew well on most of the media tested, producing yellow-white to deep brown substrate mycelium and white aerial mycelium. Light gray to deep brown diffusible pigments were produced. The substrate mycelium was well developed and fragmented with age; the aerial mycelium produced long, straight to flexuous spore chains with non-motile, smooth-surfaced, rod-shaped spores on them. The strain grew in the presence of 1-15% (w/v) total salts (optimum, 3-5%) and at pH 6.0-10.5 (optimum, pH 8.5) and 10-45 degrees C (optimum, 30 degrees C). Whole-cell hydrolysates of strain YIM 90022(T) contained meso-diaminopimelic acid and no diagnostic sugars. The predominant menaquinones were MK-10(H(4)), MK-9(H(8)), MK-10(H(6)) and MK-10(H(8)). Polar lipids comprised diphosphatidylglycerol, phosphatidylcholine, phosphatidylglycerol and phosphatidylmethylethanolamine. The major cellular fatty acids were iso-C(16:0), anteiso-C(17:0), 10-methyl-C(18:0) and 10-methyl-C(17:0). The DNA G + C content of strain YIM 90022(T) was 71.5 mol%. The combination of phylogenetic analysis, DNA-DNA relatedness data, phenotypic characteristics and chemotaxonomic data supported the suggestion that strain YIM 90022(T) represents a new species of the genus Nocardiopsis, for which the name Nocardiopsis terrae sp. nov. is proposed. The type strain is YIM 90022(T) (=CCTCC AA 208011(T) =KCTC 19431(T)).
一株革兰氏阳性、中度嗜盐、兼性嗜碱、过氧化氢酶和氧化酶阳性、需氧、丝状放线菌菌株，被命名为 YIM 90022(T)，从中国西北部柴达木盆地采集的盐土中分离得到。基于 16S rRNA 基因序列的系统发育分析表明，新分离株是诺卡氏菌属的成员，与诺卡氏菌属成员的模式菌株的序列相似性在 95.1-98.7%之间。该菌株的表型和化学分类特性也表明，菌株 YIM 90022(T) 是诺卡氏菌属的成员。该菌株在大多数测试的培养基上生长良好，产生黄白色至深棕色基质菌丝和白色气生菌丝。产生浅灰色至深棕色可扩散色素。基质菌丝发育良好，随年龄增长而碎片化；气生菌丝产生长而直的、弯曲的孢子链，其上有不运动的、光滑表面的杆状孢子。该菌株在 1-15%（w/v）总盐（最佳 3-5%）和 pH 6.0-10.5（最佳 pH 8.5）和 10-45°C（最佳 30°C）的条件下生长。菌株 YIM 90022(T) 的全细胞水解物含有 meso-二氨基庚二酸，没有诊断性糖。主要的menaquinones 是 MK-10(H(4))、MK-9(H(8))、MK-10(H(6))和 MK-10(H(8))。极性脂由双磷脂酰甘油、磷脂酰胆碱、磷脂酰甘油和磷脂酰甲乙醇胺组成。主要细胞脂肪酸是 iso-C(16:0)、anteiso-C(17:0)、10-甲基-C(18:0)和 10-甲基-C(17:0)。菌株 YIM 90022(T) 的 DNA G+C 含量为 71.5 mol%。系统发育分析、DNA-DNA 相关性数据、表型特征和化学分类数据的结合表明，菌株 YIM 90022(T) 代表了诺卡氏菌属的一个新种，建议将其命名为 Nocardiopsis terrae sp. nov.。模式菌株是 YIM 90022(T)=CCTCC AA 208011(T)=KCTC 19431(T)。
