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亚种。1.0207胞外多糖通过Keap1/Nrf2途径减轻过氧化氢诱导的IPEC-J2细胞氧化应激损伤。

subsp. 1.0207 Exopolysaccharides Attenuate Hydrogen Peroxide-Induced Oxidative Stress Damage in IPEC-J2 Cells through the Keap1/Nrf2 Pathway.

作者信息

Liu Deyu, Yue Yingxue, Ping Lijun, Sun Cuicui, Zheng Tingting, Cheng Yang, Huo Guicheng, Li Bailiang

机构信息

Food College, Northeast Agricultural University, Harbin 150030, China.

Key Laboratory of Dairy Science, Ministry of Education, Northeast Agricultural University, Harbin 150030, China.

出版信息

Antioxidants (Basel). 2024 Sep 23;13(9):1150. doi: 10.3390/antiox13091150.

DOI:10.3390/antiox13091150
PMID:39334809
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11429245/
Abstract

subsp. () is one of the most commonly employed in the food industry. Exopolysaccharides (EPS) of , which are known to exhibit probiotic properties, are secondary metabolites produced during the growth of . This study identified the structure of the EPS produced by 1.0207 and investigated the mitigation of 1.0207 EPS on HO-induced oxidative stress in IPEC-J2 cells. 1.0207 EPS consisted of glucose and galactose and possessed a molecular weight of 4.06 × 10 Da. 1.0207 EPS exhibited notable scavenging capacity against DPPH, hydroxyl radicals, superoxide anions, and ABTS radicals. Additionally, 1.0207 EPS enhanced cell proliferation, reduced intracellular reactive oxygen species (ROS) accumulation, increased activity of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), catalase (CAT), and total antioxidant capacity (T-AOC) elevated the relative expression of CAT, SOD, HO-1, NQO1, ZO-1, and Occludin genes. Moreover, 1.0207 EPS improved the expression of Nrf2, Nrf2, Nrf2/Nrf2, and Bcl-2 proteins, while decreasing the expression of Keap1, Caspase3, and Bax proteins, with the best effect at a concentration of 100 μg/mL. 1.0207 EPS mitigated HO-induced oxidative stress injury in IPEC-J2 cells by activating the Keap1/Nrf2 pathway. Meanwhile, 1.0207 EPS exhibited the potential to decrease apoptosis and restore the integrity of the gut barrier. The findings establish a theoretical foundation for the development and application of 1.0207 and its EPS.

摘要

亚种（）是食品工业中最常用的[具体内容缺失]之一。已知具有益生菌特性的[具体名称缺失]胞外多糖（EPS）是[具体名称缺失]生长过程中产生的次级代谢产物。本研究确定了[具体名称缺失]1.0207产生的EPS的结构，并研究了1.0207 EPS对HO诱导的IPEC - J2细胞氧化应激的缓解作用。1.0207 EPS由葡萄糖和半乳糖组成，分子量为4.06×10道尔顿。1.0207 EPS对DPPH、羟基自由基、超氧阴离子和ABTS自由基具有显著的清除能力。此外，1.0207 EPS促进细胞增殖，减少细胞内活性氧（ROS）积累，增加超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH - Px）、过氧化氢酶（CAT）的活性以及总抗氧化能力（T - AOC），提高CAT、SOD、HO - 1、NQO1、ZO - 1和闭合蛋白基因的相对表达。此外，1.0207 EPS改善了Nrf2、Nrf2、Nrf2/Nrf2和Bcl - 2蛋白的表达，同时降低了Keap1、Caspase3和Bax蛋白的表达，在浓度为100μg/mL时效果最佳。1.0207 EPS通过激活Keap1/Nrf2途径减轻HO诱导的IPEC - J2细胞氧化应激损伤。同时，1.0207 EPS具有降低细胞凋亡和恢复肠道屏障完整性的潜力。这些发现为1.0207及其EPS的开发和应用奠定了理论基础。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/774e/11429245/b37a5b2d19f7/antioxidants-13-01150-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/774e/11429245/39e7e90d1e3a/antioxidants-13-01150-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/774e/11429245/5714a2b6e284/antioxidants-13-01150-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/774e/11429245/b3c107ae8f75/antioxidants-13-01150-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/774e/11429245/df1e3f4c01b8/antioxidants-13-01150-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/774e/11429245/7591d72f6e5c/antioxidants-13-01150-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/774e/11429245/9dc0b002b378/antioxidants-13-01150-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/774e/11429245/b37a5b2d19f7/antioxidants-13-01150-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/774e/11429245/39e7e90d1e3a/antioxidants-13-01150-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/774e/11429245/5714a2b6e284/antioxidants-13-01150-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/774e/11429245/b3c107ae8f75/antioxidants-13-01150-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/774e/11429245/df1e3f4c01b8/antioxidants-13-01150-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/774e/11429245/7591d72f6e5c/antioxidants-13-01150-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/774e/11429245/9dc0b002b378/antioxidants-13-01150-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/774e/11429245/b37a5b2d19f7/antioxidants-13-01150-g007.jpg

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