subsp. 1.0207 Exopolysaccharides Attenuate Hydrogen Peroxide-Induced Oxidative Stress Damage in IPEC-J2 Cells through the Keap1/Nrf2 Pathway.

subsp. () is one of the most commonly employed in the food industry. Exopolysaccharides (EPS) of , which are known to exhibit probiotic properties, are secondary metabolites produced during the growth of . This study identified the structure of the EPS produced by 1.0207 and investigated the mitigation of 1.0207 EPS on HO-induced oxidative stress in IPEC-J2 cells. 1.0207 EPS consisted of glucose and galactose and possessed a molecular weight of 4.06 × 10 Da. 1.0207 EPS exhibited notable scavenging capacity against DPPH, hydroxyl radicals, superoxide anions, and ABTS radicals. Additionally, 1.0207 EPS enhanced cell proliferation, reduced intracellular reactive oxygen species (ROS) accumulation, increased activity of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), catalase (CAT), and total antioxidant capacity (T-AOC) elevated the relative expression of CAT, SOD, HO-1, NQO1, ZO-1, and Occludin genes. Moreover, 1.0207 EPS improved the expression of Nrf2, Nrf2, Nrf2/Nrf2, and Bcl-2 proteins, while decreasing the expression of Keap1, Caspase3, and Bax proteins, with the best effect at a concentration of 100 μg/mL. 1.0207 EPS mitigated HO-induced oxidative stress injury in IPEC-J2 cells by activating the Keap1/Nrf2 pathway. Meanwhile, 1.0207 EPS exhibited the potential to decrease apoptosis and restore the integrity of the gut barrier. The findings establish a theoretical foundation for the development and application of 1.0207 and its EPS.