Tang Zizhong, Lin Wenjie, Chen Yusheng, Feng Shiling, Qin Yihan, Xiao Yirong, Chen Hong, Liu Yuntao, Chen Hui, Bu Tongliang, Li Qinfeng, Cai Yi, Yao Huipeng, Ding Chunbang
College of Life Sciences, Sichuan Agricultural University, Ya'an, China.
Sichuan Agricultural University Hospital, Sichuan Agricultural University, Ya'an, China.
Front Nutr. 2022 Jun 9;9:911310. doi: 10.3389/fnut.2022.911310. eCollection 2022.
The polysaccharides from were extracted by ultrasonically-assisted enzymatic extraction (UAEE). Response surface analysis was used to determine the optimum parameters as follows: addition of enzymes, 0.71%; extraction temperature, 60°C; extraction time, 18 min; liquid-solid ratio, 46:1 (mL/g). The extraction yield of polysaccharide was 3.66 ± 0.87%. A novel polysaccharide fraction (JCH-a1) from was extracted and then purified by cellulose DEAE-32 and Sephadex G-100 anion exchange chromatography. The analysis results showed that the molar ratio of galactose, glucose, and mannose in JCH-a1 cells (60.7 kDa) was 0.89:1:0.39. JCH-a1 with a triple helix contains more α-glycosides and has strong thermal stability. Moreover, JCH-a1 showed strong antioxidant activity and acted as a strong inhibitor of α-glucosidase . In addition, JCH-a1 can prolong the lifespan of . The present study might provide a basis for further study of JCH-a1 as an antioxidant and hypoglycemic food or drug.
采用超声辅助酶提取法（UAEE）从[具体来源]中提取多糖。通过响应面分析确定最佳参数如下：酶添加量0.71%；提取温度60℃；提取时间18分钟；液固比46:1（mL/g）。多糖的提取率为3.66±0.87%。从[具体来源]中提取了一种新型多糖组分（JCH-a1），然后通过纤维素DEAE-32和Sephadex G-100阴离子交换色谱进行纯化。分析结果表明，JCH-a1细胞（60.7 kDa）中半乳糖、葡萄糖和甘露糖的摩尔比为0.89:1:0.39。具有三螺旋结构的JCH-a1含有更多的α-糖苷，具有较强的热稳定性。此外，JCH-a1表现出较强的抗氧化活性，是一种强效的α-葡萄糖苷酶抑制剂。此外，JCH-a1可以延长[具体对象]的寿命。本研究可能为进一步研究JCH-a1作为抗氧化和降血糖食品或药物提供依据。
