采用鲁米诺/溶解氧化学发光体系测定辣椒粉中的苏丹红 IV。

Determination of Sudan IV in hot chilli powder with luminol/dissolved oxygen chemiluminescence system.

机构信息

Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of Ministry of Education, College of Chemistry and Materials Science, Northwest University, Xi'an, 710069, China.

出版信息

J Sci Food Agric. 2010 Jan 30;90(2):338-42. doi: 10.1002/jsfa.3822.

DOI:10.1002/jsfa.3822

PMID:20355051

Abstract

BACKGROUND

A green, simple and sensitive flow injection chemiluminescence (CL) procedure is proposed for the determination of Sudan dyes. The method is based on the finding that Sudan I, II, III and IV markedly enhance the CL intensity of the luminol/dissolved oxygen reaction.

RESULTS

The increment in CL intensity was proportional to the concentration of Sudan I, II, III and IV, giving calibration graphs linear over the ranges 0.007-1, 0.5-30, 1-10 and 0.7-300 ng mL(-1) respectively (R(2) >or= 0.9981), with limits of detection of 0.002, 0.2, 0.3 and 0.2 ng mL(-1) respectively. At a flow rate of 2 mL min(-1), complete determination of Sudan dyes, including sampling and washing, could be accomplished in 40 s, with relative standard deviations of less than 5% (n = 7).

CONCLUSION

The proposed method was successfully applied to the determination of Sudan IV in contaminated hot chilli powder, with recoveries ranging from 89.3 to 108.4%. The possible mechanism of enhancement of the luminol/dissolved oxygen CL reaction by Sudan IV can be attributed to the acceleration of electron transfer. Compared with other procedures, the proposed CL method offers the highest sensitivity and the least reagent consumption for the determination of Sudan IV.

摘要

背景

提出了一种绿色、简单、灵敏的流动注射化学发光（CL）程序，用于测定苏丹染料。该方法基于苏丹 I、II、III 和 IV 显著增强鲁米诺/溶解氧反应的 CL 强度这一发现。

结果

CL 强度的增量与苏丹 I、II、III 和 IV 的浓度成正比，分别给出了在 0.007-1、0.5-30、1-10 和 0.7-300ng mL(-1)范围内的校准曲线线性（R(2)≥0.9981），检测限分别为 0.002、0.2、0.3 和 0.2ng mL(-1)。在 2 mL min(-1)的流速下，苏丹染料的完全测定（包括采样和洗涤）可以在 40 s 内完成，相对标准偏差小于 5%（n=7）。