Department of Analytical Chemistry, Faculty of Pharmacy, Cairo University, Kaser El-Aini Street, ET 11562, Cairo, Egypt.
Drug Test Anal. 2009 May;1(5):228-33. doi: 10.1002/dta.37.
Three multivariate calibration methods, including classical least square with nonzero intercept (CLS), principal component regression (PCR) and partial least square (PLS), have been used for the determination of pyritinol dihydrochloride in the presence of its degradation product. The CLS, PCR and PLS techniques are useful in spectral analysis because the simultaneous inclusion of many spectral wavelengths instead of the single wavelength used in derivative spectrophotometry has greatly improved the precision and predictive abilities of these multivariate calibrations. A training set was constructed for the mixture and the best model was used for the prediction of the concentration of the selected drug. The proposed procedures were applied successfully in the determination of pyritinol dihydrochloride in laboratory-prepared mixtures and in commercial preparations. Pyritinol dihydrochloride was analysed with mean accuracies 99.99 +/- 0.905, 99.91 +/- 0.966 and 99.92 +/- 0.962 using the CLS, PCR and PLS methods respectively. The validity of the proposed methods was assessed using the standard addition technique. The proposed procedures were found to be rapid and simple and required no preliminary separation. They can therefore be used for the routine analysis of pyritinol dihydrochloride in quality-control laboratories.
三种多元校正方法，包括带有非零截距的经典最小二乘法（CLS）、主成分回归（PCR）和偏最小二乘法（PLS），已被用于在其降解产物存在下测定盐酸吡硫醇。CLS、PCR 和 PLS 技术在光谱分析中很有用，因为同时包含许多光谱波长，而不是导数分光光度法中使用的单个波长，大大提高了这些多元校正的精度和预测能力。为混合物构建了一个训练集，并使用最佳模型对所选药物的浓度进行预测。所提出的程序已成功应用于实验室制备混合物和商业制剂中盐酸吡硫醇的测定。使用 CLS、PCR 和 PLS 方法分别分析盐酸吡硫醇，平均准确度为 99.99 ± 0.905、99.91 ± 0.966 和 99.92 ± 0.962。使用标准添加技术评估了所提出方法的有效性。所提出的程序快速简单，无需初步分离。因此，它们可用于质量控制实验室中盐酸吡硫醇的常规分析。
