聚乙二醇化色谱法：在接枝智能生物相容聚合物的硅胶整体柱上进行高效生物分离。

PEGylated Chromatography: Efficient Bioseparation on Silica Monoliths Grafted with Smart Biocompatible Polymers.

机构信息

Colloid Chemistry Department, Max-Planck Institute of Colloids and Interfaces, Scientific Campus Golm, 14476 Potsdam, Germany.

出版信息

ACS Appl Mater Interfaces. 2009 Sep;1(9):1869-72. doi: 10.1021/am900461a.

DOI:10.1021/am900461a

PMID:20355808

Abstract

Novel oligo(ethylene glycol)-based thermoresponsive stationary phases have been studied for the separation of bioanalytes. Well-defined copolymers of (2-methoxyethoxy)ethyl methacrylate and oligo(ethylene glycol) methacrylate were synthesized by atom-transfer radical polymerization in the presence of an N-succinimidyl-functionalized initiator. The reactive chain ends of these copolymers were then reacted with amino-functionalized silica monoliths. The formed composites were studied as chromatography materials. For instance, it was demonstrated that thermoresponsive oligo(ethylene glycol)-based stationary phases allow rapid, efficient separation of steroid and protein mixtures in pure water under isocratic high-performance liquid chromatographic elution.

摘要

新型聚（乙二醇）基温敏固定相已被用于生物分析物的分离研究。通过原子转移自由基聚合，在 N-琥珀酰亚胺基功能化引发剂的存在下合成了具有明确结构的（2-甲氧基乙氧基）乙基甲基丙烯酸酯和聚乙二醇甲基丙烯酸酯共聚物。然后，这些共聚物的活性链末端与氨基功能化的硅胶整体柱反应。形成的复合材料被用作色谱材料。例如，已经证明，温敏聚（乙二醇）基固定相可以在等度高效液相色谱洗脱条件下，在纯水中快速、有效地分离甾体和蛋白质混合物。