Department of Microbiology and Immunology, University of Michigan Medical School, 5736 Medical Science Building II, 1150 W. Medical Center Drive, Ann Arbor, MI 48109-0620, USA.
Biol Cell. 2010 Mar 25;102(6):335-50. doi: 10.1042/BC20090165.
Advances in cell biology and biophysics revealed that cellular membranes consist of multiple microdomains with specific sets of components such as lipid rafts and TEMs (tetraspanin-enriched microdomains). An increasing number of enveloped viruses have been shown to utilize these microdomains during their assembly. Among them, association of HIV-1 (HIV type 1) and other retroviruses with lipid rafts and TEMs within the PM (plasma membrane) is well documented. In this review, I describe our current knowledge on interrelationships between PM microdomain organization and the HIV-1 particle assembly process. Microdomain association during virus particle assembly may also modulate subsequent virus spread. Potential roles played by microdomains will be discussed with regard to two post-assembly events, i.e., inhibition of virus release by a raft-associated protein BST-2/tetherin and cell-to-cell HIV-1 transmission at virological synapses.
细胞生物学和生物物理学的进展表明，细胞膜由多个具有特定成分（如脂筏和 TEMs（四跨膜蛋白丰富微区））的微区组成。越来越多的包膜病毒在组装过程中被证明利用了这些微区。其中，HIV-1（HIV 型 1）和其他逆转录病毒与 PM（质膜）内的脂筏和 TEMs 的关联已有充分的文献记载。在这篇综述中，我描述了我们目前对 PM 微区组织与 HIV-1 颗粒组装过程之间相互关系的了解。病毒颗粒组装过程中的微区关联也可能调节随后的病毒传播。将讨论微区所发挥的潜在作用，涉及两个组装后的事件，即：与脂筏相关的蛋白 BST-2/ tetherin 抑制病毒释放，以及病毒突触处的细胞间 HIV-1 传播。
