Department of Genetic Engineering, Cheongju University, Cheongju 360-764, Korea.
BMB Rep. 2010 Mar;43(3):219-24. doi: 10.5483/bmbrep.2010.43.3.219.
Lipid peroxidation is known to be an important factor in the pathologies of many diseases associated with oxidative stress. We assessed the lipid peroxidation induced by the reaction of ferritin with H2O2. When linoleic acid micelles or phosphatidyl choline liposomes were incubated with ferritin and H2O2, lipid peroxidation increased in the presence of ferritin and H2O2 in a concentration-dependent manner. The hydroxyl radical scavengers, azide and thiourea, prevented lipid peroxidation induced by the ferritin/H2O2 system. The iron specific chelator desferoxamine also prevented ferritin/H2O2 systemmediated lipid peroxidation. These results demonstrate the possible role of iron in ferritin/H2O2 system-mediated lipid peroxidation. Carnosine is involved in many cellular defense processes, including free radical detoxification. In this study, carnosine, homocarnosine, and anserine were shown to significantly prevent ferritin/H2O2 system-mediated lipid peroxidation and also inhibited the free radical-generation activity of ferritin. These results indicated that carnosine and related compounds may prevent ferritin/H2O2 system-mediated lipid peroxidation via free radical scavenging.
脂质过氧化是许多与氧化应激相关疾病的病理学中的一个重要因素。我们评估了铁蛋白与 H2O2 反应引起的脂质过氧化。当亚油酸胶束或磷脂酰胆碱脂质体与铁蛋白和 H2O2 孵育时，脂质过氧化在铁蛋白和 H2O2 的存在下以浓度依赖性方式增加。羟基自由基清除剂叠氮化物和硫脲可预防铁蛋白/H2O2 系统诱导的脂质过氧化。铁特异性螯合剂去铁胺也可预防铁蛋白/H2O2 系统介导的脂质过氧化。这些结果表明铁可能在铁蛋白/H2O2 系统介导的脂质过氧化中起作用。肌肽参与许多细胞防御过程，包括自由基解毒。在这项研究中，肌肽、同型肌肽和鹅肌肽显著预防了铁蛋白/H2O2 系统介导的脂质过氧化，并抑制了铁蛋白的自由基生成活性。这些结果表明，肌肽和相关化合物可能通过自由基清除来预防铁蛋白/H2O2 系统介导的脂质过氧化。
