Division of Maternal-Fetal Medicine, Department of Obstetrics and Gynecology, University of Michigan Medical School, Ann Arbor, MI.
Am J Obstet Gynecol. 2010 May;202(5):469.e1-6. doi: 10.1016/j.ajog.2010.01.076. Epub 2010 Mar 31.
Lipopolysaccharide pretreatment potentiates hypoxic ischemic injury. We hypothesized that docosahexaenoic acid pretreatment would improve function and reduce brain volume loss in this rat model of perinatal brain injury and inflammation.
Seven-day-old rats were divided into 3 groups: intraperitoneal docosahexaenoic acid 1 mg/kg and lipopolysaccharide 0.1 mg/kg, 25% albumin and lipopolysaccharide, and normal saline. Injections were given 2.5 hours before carotid ligation, followed by 90 minutes 8% O2. Rats underwent sensorimotor function testing and brain volume loss assessment on postnatal day 14.
Docosahexaenoic acid pretreatment improved vibrissae forepaw placing scores compared with albumin/lipopolysaccharide (mean+/-standard deviation weighted score/20: 17.72+/-0.92 docosahexaenoic acid/lipopolysaccharide vs 13.83+/-0.82 albumin/lipopolysaccharide; P<.007). Albumin/lipopolysaccharide rats scores were worse than those of the normal saline/normal saline rats (13.83+/-0.82 vs 17.21+/-0.71; P=.076). No significant differences in brain volume loss were observed among groups.
Lipopolysaccharide inflammatory stimulation in conjunction with hypoxic ischemic resulted in poorer function than hypoxic ischemic alone. Docosahexaenoic acid pretreatment had significantly improved function in neonatal rats exposed to lipopolysaccharide and hypoxic ischemic.
脂多糖预处理会增强缺氧缺血性损伤。我们假设二十二碳六烯酸预处理会改善该围生期脑损伤和炎症大鼠模型的功能并减少脑体积损失。
7 日龄大鼠分为 3 组：腹腔内二十二碳六烯酸 1mg/kg 和脂多糖 0.1mg/kg、25%白蛋白和脂多糖以及生理盐水。在颈总动脉结扎前 2.5 小时给予注射，随后进行 90 分钟 8%O2 缺氧。大鼠在生后第 14 天进行感觉运动功能测试和脑体积损失评估。
二十二碳六烯酸预处理与白蛋白/脂多糖组相比，提高了触须前肢放置评分（加权评分/20 的平均+/-标准差：17.72+/-0.92 二十二碳六烯酸/脂多糖与 13.83+/-0.82 白蛋白/脂多糖；P<.007）。白蛋白/脂多糖组大鼠的评分比生理盐水/生理盐水组差（13.83+/-0.82 与 17.21+/-0.71；P=.076）。各组间脑体积损失无显著差异。
脂多糖炎症刺激与缺氧缺血相结合导致的功能比单纯缺氧缺血差。在接受脂多糖和缺氧缺血的新生大鼠中，二十二碳六烯酸预处理显著改善了功能。
