Berman Deborah R, Mozurkewich Ellen, Liu Yiqing, Barks John
Division of Maternal-Fetal Medicine, Department of Obstetrics and Gynecology, University of Michigan Medical School, Ann Arbor, MI, USA.
Am J Obstet Gynecol. 2009 Mar;200(3):305.e1-6. doi: 10.1016/j.ajog.2009.01.020.
We hypothesized that pretreatment with docosahexaenoic acid (DHA), a potentially neuroprotective polyunsaturated fatty acid, would improve function and reduce brain damage in a rat model of perinatal hypoxia-ischemia.
Seven-day-old rats were divided into 3 treatment groups that received intraperitoneal injections of DHA 1, 2.5, or 5 mg/kg as DHA-albumin complex and 3 controls that received 25% albumin, saline, or no injection. Subsequently, rats underwent right carotid ligation followed by 90 minutes of 8% oxygen. Rats underwent sensorimotor testing (vibrissae-stimulated forepaw placing) and morphometric assessment of right-sided tissue loss on postnatal day 14.
DHA pretreatment improved forepaw placing response to near-normal levels (9.5 +/- 0.9 treatment vs 7.1 +/- 2.2 controls; normal = 10; P < .0001). DHA attenuated hemisphere damage compared with controls (P = .0155), with particular benefit in the hippocampus with 1 mg/kg (38% protection vs albumin controls).
DHA pretreatment improves functional outcome and reduces volume loss after hypoxia-ischemia in neonatal rats.
我们推测，用二十二碳六烯酸（DHA）进行预处理，这是一种具有潜在神经保护作用的多不饱和脂肪酸，在围产期缺氧缺血大鼠模型中可改善功能并减少脑损伤。
将7日龄大鼠分为3个治疗组，分别腹腔注射1、2.5或5 mg/kg的DHA - 白蛋白复合物，以及3个对照组，分别注射25%白蛋白、生理盐水或不注射。随后，对大鼠进行右侧颈动脉结扎，然后吸入8%氧气90分钟。在出生后第14天对大鼠进行感觉运动测试（触须刺激前爪放置）和右侧组织损失的形态学评估。
DHA预处理将前爪放置反应改善至接近正常水平（治疗组为9.5±0.9，对照组为7.1±2.2；正常为10；P <.0001）。与对照组相比，DHA减轻了半球损伤（P = 0.0155），1 mg/kg的DHA对海马体有特别的益处（与白蛋白对照组相比有38%的保护作用）。
DHA预处理可改善新生大鼠缺氧缺血后的功能结局并减少体积损失。
