Interdisciplinary Program of Integrated Biotechnology, Sogang University, 1 Sinsoo-Dong, Mapo-Gu, Seoul 121-742, Republic of Korea.
Bioelectrochemistry. 2010 Oct;79(2):153-61. doi: 10.1016/j.bioelechem.2010.02.004. Epub 2010 Mar 1.
Peptide nucleic acid (PNA) is the modified DNA or DNA analogue with a neutral peptide backbone instead of a negatively charged sugar phosphate. PNA exhibits chemical stability, resistant to enzymatic degradation inside living cell, recognizing specific sequences of nucleic acid, formation of stable hybrid complexes like PNA/DNA/PNA triplex, strand invasion, extraordinary thermal stability and ionic strength, and unique hybridization relative to nucleic acids. These unique physicobiochemical properties of PNA enable a new mode of detection, which is a faster and more reliable analytical process and finds applications in the molecular diagnostics and pharmaceutical fields. Besides, a variety of unique characteristic features, PNAs replace DNA as a probe for biomolecular tool in the molecular genetic diagnostics, cytogenetics, and various pharmaceutical potentials as well as for the development of sensors/arrays/chips and many more investigation purposes. This review paper discusses the various current aspects related with PNAs, making a new hot device in the commercial applications like nanobiosensor arrays.
肽核酸（PNA）是一种经过修饰的 DNA 或 DNA 类似物，其具有中性的肽骨架而不是带负电荷的糖磷酸。PNA 具有化学稳定性，能够抵抗细胞内的酶降解，识别特定的核酸序列，形成类似于 PNA/DNA/PNA 三聚体的稳定杂交复合物、链入侵、非凡的热稳定性和离子强度，以及与核酸独特的杂交方式。PNA 的这些独特的物理化学特性使其能够实现新的检测模式，这种检测模式具有更快、更可靠的分析过程，并在分子诊断和制药领域得到了应用。此外，PNA 还具有多种独特的特性，可替代 DNA 作为分子遗传学诊断、细胞遗传学以及各种药物潜力中的生物分子工具的探针，也可用于传感器/阵列/芯片的开发以及更多的研究目的。本文综述了与 PNA 相关的各种当前方面，使其成为商业应用（如纳米生物传感器阵列）中的新型热门器件。
