大肠杆菌电子传递链中b型细胞色素相对于泛醌的顺序。
Sequence of b cytochromes relative to ubiquinone in the electron transport chain of Escherichia coli.
作者信息
Downie J A, Cox G B
出版信息
J Bacteriol. 1978 Feb;133(2):477-84. doi: 10.1128/jb.133.2.477-484.1978.
Abstract
A ubiquinone-deficient mutant, carrying mutations in two genes affecting ubiquinone biosynthesis, has been used, in comparison with a normal strain, to determine the sequence of some of the components of the electron transport chain of Escherichia coli. The amounts of cytochromes reduced during aerobic steady-state conditions were estimated by comparing low-temperature difference spectra of normal or ubiquinone-deficient membranes with either D-lactate or reduced nicotinamide adenine dinucleotide as substrate. From the amounts of cytochromes reduced it was concluded that ubiquinone functions at two sites, one site being between the dehydrogenases and cytochromes and the second site being after cytochromes b562 and b556 but before cytochromes b558, d, and o. The scheme proposed is discussed in relation to the Mitchell protonmotive ubiquinone cycle.
摘要
一个泛醌缺陷型突变体，其两个影响泛醌生物合成的基因发生了突变，已被用于与正常菌株比较，以确定大肠杆菌电子传递链某些组分的顺序。通过比较正常或泛醌缺陷型膜在以D-乳酸或还原型烟酰胺腺嘌呤二核苷酸为底物时的低温差光谱，估算了有氧稳态条件下还原的细胞色素量。从还原的细胞色素量得出结论，泛醌在两个位点起作用，一个位点在脱氢酶和细胞色素之间，第二个位点在细胞色素b562和b556之后但在细胞色素b558、d和o之前。所提出的方案结合米切尔质子动力泛醌循环进行了讨论。
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