PITTARD J
J Bacteriol. 1965 Mar;89(3):680-6. doi: 10.1128/jb.89.3.680-686.1965.
Pittard, James (Yale University, New Haven, Conn.). Effect of integrated sex factor on the transduction of chromosomal genes in Escherichia coli. J. Bacteriol. 89:680-686. 1965.-Generalized transducing phage P1kc can contransduce the markers tna and ilv in Escherichia coli K-12 at a frequency of approximately 25%. When sex factor is integrated into the donor chromosome between tna and ilv, as in Hfr AB313, the entire sequence tna F ilv is not cotransduced at a detectable frequency, nor is F cotransduced with either tna or ilv. The frequency with which ilv is transduced from Hfr AB313 to female recipients is approximately one-eighth the frequency obtained when either F(+) or female strains are used as donors. This reduction of transduction frequency does not occur if the recipient is an Hfr male similar to AB313. The reduction is attributed to pairing difficulties which occur when the transduced fragment carrying the ilv genes also carries sex-factor material. When ilv(+) is transduced from a female to an ilv(-) derivative of AB313, 70% of the transductants are converted to F(+) males. A model is proposed to account for all of these observations.
皮塔德，詹姆斯（耶鲁大学，康涅狄格州纽黑文）。整合性因子对大肠杆菌染色体基因转导的影响。《细菌学杂志》89:680 - 686。1965年。- 广义转导噬菌体P1kc能以约25%的频率共转导大肠杆菌K - 12中的标记物tna和ilv。当性因子整合到供体染色体上tna和ilv之间时，如在Hfr AB313中，整个序列tna F ilv不会以可检测到的频率被共转导，F也不会与tna或ilv共转导。从Hfr AB313向雌性受体转导ilv的频率约为以F(+)或雌性菌株作为供体时所获频率的八分之一。如果受体是与AB313相似的Hfr雄性，则不会出现这种转导频率的降低。这种降低归因于当携带ilv基因的转导片段也携带性因子物质时发生的配对困难。当ilv(+)从雌性转导到AB313的ilv(-)衍生物时，70%的转导子会转变为F(+)雄性。提出了一个模型来解释所有这些观察结果。
