Department of Neurosurgery, Sanglah General Hospital, School of Medicine, Udayana University, Denpasar, Bali, Indonesia.
Singapore Med J. 2010 Feb;51(2):105-9.
Clinical studies have shown that the incidence of early post-traumatic seizures is higher in children than in adults. It has been proposed that iron-induced lipid peroxidation plays an important role in the development of epileptogenic foci. This study examined some of the hypothesised reasons for the difference in the incidence of early post-traumatic seizures between children and adults.
12 young rats and 12 adult rats were randomised into four groups. Groups 1 and 2 were control groups, comprising six young rats and six adult rats, respectively, and they were administered an intracortical injection of saline. Groups 3 and 4 were injury groups, comprising six young rats and six adult rats, respectively, and they were administered an intracortical injection of FeCl3. All the rats were observed for six hours post-injection for the occurrence of seizures, and were then killed. The injected hemispheres were extirpated and tested for the malondialdehyde (MDA) levels and superoxide dismutase (SOD) activity as indices of oxidative damage.
Seizures were observed only in Group 3. Increased MDA levels and decreased SOD activity were observed in Group 3 (ANOVA, p-value is less than 0.001). Increased MDA levels and decreased SOD activity were significantly higher in rats with seizures (Group 3) than in those without seizures (independent t-test, p-value is less than 0.001).
Different levels of lipid peroxidation induced by an intracortical ferric chloride injection may account for the different incidence rates of seizures between young and adult rats.
临床研究表明，儿童早期创伤后癫痫发作的发生率高于成人。有人提出，铁诱导的脂质过氧化在致痫灶的形成中起重要作用。本研究探讨了儿童和成人早期创伤后癫痫发作发生率差异的一些假设原因。
将 12 只幼鼠和 12 只成年鼠随机分为四组。第 1 组和第 2 组为对照组，每组包括 6 只幼鼠和 6 只成年鼠，分别给予皮质内注射生理盐水。第 3 组和第 4 组为损伤组，每组包括 6 只幼鼠和 6 只成年鼠，分别给予皮质内注射 FeCl3。所有大鼠在注射后 6 小时内观察癫痫发作的发生情况，然后处死。取出注射的半球，检测丙二醛（MDA）水平和超氧化物歧化酶（SOD）活性作为氧化损伤的指标。
仅在第 3 组观察到癫痫发作。第 3 组 MDA 水平升高，SOD 活性降低（ANOVA，p 值小于 0.001）。有癫痫发作的大鼠（第 3 组）MDA 水平升高和 SOD 活性降低明显高于无癫痫发作的大鼠（独立 t 检验，p 值小于 0.001）。
皮质内氯化铁注射引起的脂质过氧化水平不同，可能是幼鼠和成年鼠癫痫发作发生率不同的原因。
