新型开管毛细管电色谱中的涂层作为生物膜模型。
New capillary coatings in open tubular CEC as models for biological membranes.
机构信息
Department of Natural Sciences, Institute of Mathematics and Natural Sciences, Tallinn University, Tallinn, Estonia.
出版信息
Electrophoresis. 2010 May;31(9):1586-9. doi: 10.1002/elps.200900587.
Novel stationary phases in open tubular CEC were investigated. The coating procedure was fast and simple. The coating material contained membrane suspension of different neuronal cell lines. The performance and stability of three cell lines: human neuroblastoma SH-SY5Y, murine microglia Bv-2 and human glioma U87-MG cells were studied. The coating solution was expected to contain both membrane proteins and membrane lipids. The presence of membrane proteins was tested by Western blotting and the presence of phospholipids by the analysis of phosphorus content. The stability of the coating was estimated by monitoring the mobility of EOF over successive runs. The effects of pH, storage time and temperature on the coating stability were also studied. The results showed that the cell membrane-based coating was stable over pH range of 6.5-8.5. Coatings derived from different cells yielded similar stability and EOF mobility. Capillary coated with a membrane solution was stable over 3-day period. The same coating solution could be used for 3 weeks.
研究了用于开管 CEC 的新型固定相。涂层过程快速简单。涂层材料包含不同神经元细胞系的膜悬浮液。研究了三种细胞系的性能和稳定性：人神经母细胞瘤 SH-SY5Y、鼠小胶质细胞 Bv-2 和人神经胶质瘤 U87-MG 细胞。涂层溶液预计包含膜蛋白和膜脂。通过 Western blot 测试膜蛋白的存在，通过磷含量分析测试磷脂的存在。通过监测连续运行时 EOF 的迁移率来估计涂层的稳定性。还研究了 pH 值、储存时间和温度对涂层稳定性的影响。结果表明，在 pH 值为 6.5-8.5 的范围内，基于细胞膜的涂层是稳定的。来自不同细胞的涂层具有相似的稳定性和 EOF 迁移率。用膜溶液涂覆的毛细管在 3 天内稳定。相同的涂层溶液可以使用 3 周。
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