Fowler J D, Robertson C R
Department of Chemical Engineering, Stanford University, California 94305-5025.
Appl Environ Microbiol. 1991 Jan;57(1):93-101. doi: 10.1128/aem.57.1.93-101.1991.
Experiments were conducted on immobilized aggregates of Escherichia coli cells. Mechanical stress was applied by forcing a convective stream of nutrient medium through the aggregate. It was shown to be possible to maintain uniform exponential growth with this convective supply of nutrients. Analysis of effluent from the system allowed investigation of metabolic responses unambiguously attributable to mechanical stress. A reversible increase in catabolic activity was observed after an increase in mechanical stress. Changes in the level of catabolism were accompanied by an alteration in the total acid yield on glucose and in the spectrum of organic acids produced during glucose fermentation. The behavior observed here was likely due to an osmoregulatory response induced by the mechanically stressed bacteria to counteract changes in shape.
对大肠杆菌细胞的固定化聚集体进行了实验。通过迫使营养培养基的对流通过聚集体来施加机械应力。结果表明，通过这种对流营养供应能够维持均匀的指数生长。对系统流出物的分析使得能够明确研究可归因于机械应力的代谢反应。在机械应力增加后，观察到分解代谢活性有可逆增加。分解代谢水平的变化伴随着葡萄糖总酸产量以及葡萄糖发酵过程中产生的有机酸谱的改变。此处观察到的行为可能是由于机械应激细菌诱导的渗透调节反应以抵消形状变化所致。
