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通过免疫粘连血凝反应定量检测水痘-带状疱疹病毒抗体

Quantitation of antibodies to varicella-zoster virus by immune adherence hemagglutination.

作者信息

Wong C L, Castriciano S, Chernesky M A, Rawls W E

出版信息

J Clin Microbiol. 1978 Jan;7(1):6-11. doi: 10.1128/jcm.7.1.6-11.1978.

DOI:10.1128/jcm.7.1.6-11.1978

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC274847/

Abstract

Immune adherence hemagglutination was compared with the complement fixation test as a means of measuring antibodies to varicella-zoster virus. Analysis of acute- and convalescent-phase sera from patients infected with varicella-zoster or with herpes simplex virus showed the immune adherence hemagglutination test to be more sensitive than the complement fixation test, and greater cross-reactivity between the two viruses appeared to be associated with the increased sensitivity. The two assay methods were used to measure antibodies to varicella-zoster virus in 265 sera obtained from patients of different ages as well as sera from 26 patients with leukemia. There were 35 cases where antibodies were detected by immune adherence hemagglutination but not by complement fixation, whereas in five cases the converse was found. Our findings support the contention that immune adherence hemagglutination is the method of choice for detecting antibodies to varicella-zoster virus.

摘要

将免疫黏附血凝试验与补体结合试验进行比较，以作为检测水痘-带状疱疹病毒抗体的一种方法。对感染水痘-带状疱疹病毒或单纯疱疹病毒患者的急性期和恢复期血清进行分析，结果显示免疫黏附血凝试验比补体结合试验更敏感，并且两种病毒之间更大的交叉反应性似乎与敏感性增加有关。使用这两种检测方法对从不同年龄患者获得的265份血清以及26例白血病患者的血清进行水痘-带状疱疹病毒抗体检测。有35例通过免疫黏附血凝试验检测到抗体，但补体结合试验未检测到，而在5例中情况相反。我们的研究结果支持以下观点，即免疫黏附血凝试验是检测水痘-带状疱疹病毒抗体的首选方法。