Li Xiangmin, Zhou Qiaoling
Department of Emergency, Xiangya Hospital, Central South University, Changsha 410008, China.
Zhong Nan Da Xue Xue Bao Yi Xue Ban. 2010 Mar;35(3):254-61. doi: 10.3969/j.issn.1672-7347.2010.03.011.
To observe the effect of valsartan on brian ultrastructure, Klotho gene and micro-inflammatory factor [intercellular adhesion molecule-1(ICAM-1) and vascular cell adhesion molecule-l(VCAM-1)] expression in spontaneously hypertensive rat models.
Ten male spontaneously hypertensive rats of 22 weeks age were selected and randomly divided into a hypertension group and a valsartan intervention group, while another 5 Wistar-kyoto rats were set as a normal contrast group. The brain ultrastructure of the 2 groups was observed by electron microscope. The expression of micro-inflammatory factor (ICAM-1 and VCAM-1)and Klotho gene was detected with RT-PCR, immunohistochemistry, and Western blot, respectively.
The cerebral neuron damage of spontaneously hypertensive rats whose ultrastructure showed cell-pyknosis, chromatin margination and typical apoptotic body formation were alleviated after the intervention of valsartan. RT-PCR showed that the gene expression of Klotho increased while ICAM-1 and VCAM-1 decreased after valsartan intervention. Immunohistochemistry and Western blot also showed that the protein expression of Klotho increased, while ICAM-1 and VCAM-1 decreased after valsartan intervention.
Valsartan can improve the brain ultrastructure of spontaneously hypertensive rats by increasing the expression of Klotho.
观察缬沙坦对自发性高血压大鼠模型脑超微结构、Klotho基因及微炎症因子[细胞间黏附分子-1（ICAM-1）和血管细胞黏附分子-1（VCAM-1）]表达的影响。
选取10只22周龄雄性自发性高血压大鼠，随机分为高血压组和缬沙坦干预组，另设5只Wistar-京都大鼠作为正常对照组。通过电子显微镜观察两组大鼠的脑超微结构。分别采用逆转录聚合酶链反应（RT-PCR）、免疫组织化学和蛋白质免疫印迹法检测微炎症因子（ICAM-1和VCAM-1）及Klotho基因的表达。
缬沙坦干预后，自发性高血压大鼠脑神经元损伤减轻，其超微结构表现为细胞核固缩、染色质边集及典型凋亡小体形成。RT-PCR结果显示，缬沙坦干预后Klotho基因表达增加，ICAM-1和VCAM-1表达降低。免疫组织化学和蛋白质免疫印迹法结果也显示，缬沙坦干预后Klotho蛋白表达增加，ICAM-1和VCAM-1蛋白表达降低。
缬沙坦可通过增加Klotho表达改善自发性高血压大鼠的脑超微结构。
