Wu Rong, Zhou Qiaoling, Lin Shudian, Ao Xiang, Chen Xiaomiao, Yang Jinghua
Department of Nephrology, Xiangya Hospital of Central South University, Changsha 410008, China.
Zhong Nan Da Xue Xue Bao Yi Xue Ban. 2010 Feb;35(2):152-8. doi: 10.3969/j.issn.1672-7347.2010.02.011.
To observe the effect of Cordceps Sinensis (CS) on the expression of intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1) in the kidney of spontaneously hypertensive rats(SHR), and to investigate the mechanism of CS.
Male SHRs (23 week old) were randomly divided into 4 groups: a group without any treatment (Group S), a group treated with Cordceps sinensis at 4 g/(kg.d)(Group C), a group treated with fosinopril at 10 mg/(kg.d) (Group F), and a group received daily intragastric administration of CS at 4 g/(kg.d) and fosinopril at 10 mg/(kg.d) (Group CF). At the same time, 6 male WKY rats were used as normals controls. At the end of 8 weeks, all rats were sacrificed. Serum creatinine(Scr), 24 h urinary protein count, and the expression of ICAM-1 and VCAM-1 were examined by immunohistochemical technique and RT-PCR.
Compared with the WKY rats, blood pressure, 24 h urinary protein count, Scr,and the expression of ICAM-1 andVCAM-1 in the kidney of SHR significantly increased (P<0.05).Compared with Group S, blood pressure decreased after treatment by fosinopril (P<0.05). Compared with Group S, the levels of Scr, 24 h urinary protein count, and glomerular lesion were significantly reduced in the CS and/or fosinopril treatment group. The expression of ICAM-1 and VCAM-1 was significantly decreased in these groups (P<0.05).
CS may play a role in the protection and anti-fibrosis in the process of renal injury in SHR through reducing the expression of ICAM-1 and VCAM-1.
观察冬虫夏草对自发性高血压大鼠（SHR）肾脏细胞间黏附分子-1（ICAM-1）和血管细胞黏附分子-1（VCAM-1）表达的影响，并探讨其作用机制。
将23周龄雄性SHR随机分为4组：未治疗组（S组）、冬虫夏草4 g/(kg·d)治疗组（C组）、福辛普利10 mg/(kg·d)治疗组（F组）、冬虫夏草4 g/(kg·d)联合福辛普利10 mg/(kg·d)治疗组（CF组）。同时，选取6只雄性WKY大鼠作为正常对照组。8周结束时，处死所有大鼠。采用免疫组织化学技术和逆转录-聚合酶链反应检测血清肌酐（Scr）、24小时尿蛋白定量以及ICAM-1和VCAM-1的表达。
与WKY大鼠相比，SHR血压、24小时尿蛋白定量、Scr以及肾脏ICAM-1和VCAM-1表达显著升高（P<0.05）。与S组相比，福辛普利治疗后血压降低（P<0.05）。与S组相比，冬虫夏草和/或福辛普利治疗组Scr水平、24小时尿蛋白定量及肾小球病变明显减轻。这些组中ICAM-1和VCAM-1表达显著降低（P<0.05）。
冬虫夏草可能通过降低ICAM-1和VCAM-1的表达，在SHR肾损伤过程中发挥保护和抗纤维化作用。
