Department of Otolaryngology, VU University Medical Center, Amsterdam, The Netherlands.
Nat Protoc. 2010 Apr;5(4):739-43. doi: 10.1038/nprot.2010.13. Epub 2010 Mar 25.
The positron emitter zirconium-89 ((89)Zr) has very attractive properties for positron emission tomography (PET) imaging of intact monoclonal antibodies (mAbs) using immuno-PET. This protocol describes the step-by-step procedure for the facile radiolabeling of mAbs or other proteins with (89)Zr using p-isothiocyanatobenzyl-desferrioxamine (Df-Bz-NCS). First, Df-Bz-NCS is coupled to the lysine-NH(2) groups of a mAb at pH 9.0 (pre-modification), followed by purification using gel filtration. Next, the pre-modified mAb is labeled at room temperature by the addition of [(89)Zr]Zr-oxalic acid solution followed by purification using gel filtration. The entire process of pre-modification, radiolabeling and purification steps will take about 2.5 h.
正电子发射体锆-89((89)Zr) 具有非常吸引人的特性，可用于使用免疫 PET 对完整的单克隆抗体(mAb) 进行正电子发射断层扫描 (PET) 成像。本方案描述了使用对异硫氰酸苯甲基-去铁胺 (Df-Bz-NCS) 轻松对 mAb 或其他蛋白质进行 (89)Zr 放射性标记的逐步程序。首先，在 pH 9.0 下将 Df-Bz-NCS 偶联到 mAb 的赖氨酸-NH(2)基团上(预修饰)，然后通过凝胶过滤进行纯化。接下来，通过添加 [(89)Zr]Zr-草酸溶液，在室温下对预修饰的 mAb 进行标记，然后通过凝胶过滤进行纯化。预修饰、放射性标记和纯化步骤的整个过程大约需要 2.5 小时。
