Ozawa Tomoko, Wang Jingli, Hu Lily J, Bollen Andrew W, Lamborn Kathleen R, Deen Dennis F
Brain Tumor Research Center, Department of Neurological Surgery, School of Medicine, University of California, San Francisco, California 94143-0520, USA.
In Vivo. 2002 Jan-Feb;16(1):55-60.
We have developed xenografts of human glioblastoma (GBM) and established the baseline growth parameters and histopathological features of these tumors.
MATERIALS-METHODS: Cells from 4 different human GBM cell lines were injected into the right caudate-putamen of brain in athymic rats. We measured tumor weights and the estimated survival time of each rat. RESULTS-CONCLUSION: U-251 MG and U-87 MG cells produced solid intracerebral tumors with a 100% tumor take rate, while SF-767 and SF-126 cells did not grow in the brains of athymic rats. Under the conditions employed, U-87 MG tumors grew faster than U-251 MG tumors, but both types of tumors exhibited reproducible growth characteristics from animal to animal. There was heterogeneity in the growth characteristics and histologies between the 2 tumor types, indicating that these tumor models might be useful for simulating some of the heterogeneity that occurs between GBM in humans.
我们已开发出人类胶质母细胞瘤（GBM）异种移植模型，并确定了这些肿瘤的基线生长参数和组织病理学特征。
将来自4种不同人类GBM细胞系的细胞注射到无胸腺大鼠脑右侧尾状核-壳核中。我们测量了每只大鼠的肿瘤重量和估计存活时间。结果与结论：U-251 MG和U-87 MG细胞产生了脑内实体瘤，肿瘤接种成功率为100%，而SF-767和SF-126细胞在无胸腺大鼠脑中未生长。在所采用的条件下，U-87 MG肿瘤比U-251 MG肿瘤生长得更快，但两种类型的肿瘤在不同动物之间均表现出可重复的生长特征。这两种肿瘤类型在生长特征和组织学上存在异质性，表明这些肿瘤模型可能有助于模拟人类GBM之间发生的一些异质性。
