Department of Radiation Oncology, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, Massachusetts, United States of America.
PLoS One. 2010 Mar 31;5(3):e9885. doi: 10.1371/journal.pone.0009885.
Phellinus linteus (PL) mushroom possesses anti-tumor property. We previously reported that the treatment with PL caused cultured human prostate cancer cells to undergo apoptosis. To further studying the mechanisms of PL-mediated apoptosis, we performed xenograft assay, together with in vitro assays, to evaluate the effect of PL on the genesis and progression of the tumors formed from the inoculation of prostate cancer PC3 or DU145 cells. After the inoculation, nude mice were injected with PL every two days for 12 days. Although PL treatment did not prevent the formation of the inoculated tumors, the growth rate of the tumors after PL treatment was dramatically attenuated. We then tested the effect of PL on the tumors 12 days after the inoculation. After inoculated tumors reached a certain size, PL was administrated to the mice by subcutaneous injection. The histochemistry or immunochemistry analysis showed that apoptosis occurred with the activation of caspase 3 in the tumors formed by inoculating prostate cancer DU145 or PC3 cells. The data was in a good agreement with that from cultured cells. Thus, our in vivo study suggests that PL not only is able to attenuate tumor growth, but also to cause tumor regression by inducing apoptosis.
松木层孔菌（PL）蘑菇具有抗肿瘤特性。我们之前的报告表明，PL 的治疗会导致培养的人类前列腺癌细胞发生凋亡。为了进一步研究 PL 介导的凋亡机制，我们进行了异种移植实验，并结合体外实验，评估 PL 对前列腺癌 PC3 或 DU145 细胞接种形成的肿瘤发生和进展的影响。接种后，裸鼠每两天注射一次 PL，共 12 天。尽管 PL 治疗不能预防接种肿瘤的形成，但 PL 处理后肿瘤的生长速度明显减弱。然后，我们在接种后 12 天测试了 PL 对肿瘤的影响。当接种的肿瘤长到一定大小时，通过皮下注射将 PL 施用于小鼠。组织化学或免疫组织化学分析显示，接种前列腺癌 DU145 或 PC3 细胞形成的肿瘤中，caspase 3 的激活导致了细胞凋亡。这些数据与体外培养细胞的结果非常吻合。因此，我们的体内研究表明，PL 不仅能够抑制肿瘤生长，还能够通过诱导凋亡导致肿瘤消退。
