松木层孔菌多糖提取物增加线粒体膜电位并导致 THP-1 单核细胞凋亡死亡。

Phellinus linteus polysaccharide extracts increase the mitochondrial membrane potential and cause apoptotic death of THP-1 monocytes.

机构信息

Department of Bioscience, Plant Research International, Wageningen University and Research Centre, Droevendaalsesteeg 1, Wageningen 6700AA, The Netherlands.

出版信息

Chin Med. 2013 Dec 18;8(1):25. doi: 10.1186/1749-8546-8-25.

DOI:10.1186/1749-8546-8-25

PMID:24344650

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3878362/

Abstract

BACKGROUND

The differentiation resp. death of human monocytic THP-1 cells induced by polysaccharide extracts of the medicinal mushrooms Phellinus linteus, Agaricus bisporus and Agaricus brasiliensis have been studied. This study aims to identify leads for the causal effects of these mushroom components on cell differentiation and death.

METHODS

THP-1 cells were treated with different polysaccharide extracts of mushrooms and controls. Morphological effects were observed by light microscopy. Flow cytometry was applied to follow the cell differentiation by cell cycle shifts after staining with propidium iodide, changes of mitochondrial membrane potential after incubation with JC-1, and occurrence of intracellular reactive oxygen species after incubation with hydroethidine. Principal component analysis of the data was performed to evaluate the cellular effects of the different treatments.

RESULTS

P. linteus polysaccharide extracts induced dose-dependent apoptosis of THP-1 cells within 24 h, while A. bisporus and A. brasiliensis polysaccharide extracts caused differentiation into macrophages. A pure P. linteus polysaccharide had no effect. Apoptosis was inhibited by preincubating THP-1 cells with human serum. The principal component analysis revealed that P. linteus, A. bisporus and A. brasiliensis polysaccharide extracts increased reactive oxygen species production. Both A. bisporus and A. brasiliensis polysaccharide extracts decreased the mitochondrial membrane potential, while this was increased by P. linteus polysaccharide extracts.

CONCLUSIONS

P. linteus polysaccharide extracts caused apoptosis of THP-1 monocytes while A. bisporus and A. brasiliensis polysaccharide extracts caused these cells to differentiate into macrophages. The protective effects of human serum suggested that P. linteus polysaccharide extract induced apoptosis by extrinsic pathway, i.e. by binding to the TRAIL receptor. The mitochondrial membrane potential together with reactive oxygen species seems to play an important role in cell differentiation and cell death.

摘要

背景

本研究旨在探讨药用蘑菇桑黄、双孢蘑菇和巴西蘑菇多糖提取物对人单核细胞 THP-1 细胞分化和死亡的影响，以寻找这些蘑菇成分对细胞分化和死亡有因果关系的作用的线索。

方法

用不同蘑菇多糖提取物和对照品处理 THP-1 细胞。用光镜观察形态学变化。用碘化丙啶染色后通过细胞周期移位来跟踪细胞分化，用 JC-1 孵育后检测线粒体膜电位变化，用羟乙基二氢噻吩孵育后检测细胞内活性氧的产生。对数据进行主成分分析，以评估不同处理方法对细胞的影响。

结果

P. linteus 多糖提取物在 24 小时内诱导 THP-1 细胞呈剂量依赖性凋亡，而 A. bisporus 和 A. brasiliensis 多糖提取物则诱导其分化为巨噬细胞。纯 P. linteus 多糖无作用。用人血清预先孵育 THP-1 细胞可抑制凋亡。主成分分析表明，P. linteus、A. bisporus 和 A. brasiliensis 多糖提取物增加了活性氧的产生。A. bisporus 和 A. brasiliensis 多糖提取物均降低了线粒体膜电位，而 P. linteus 多糖提取物则增加了线粒体膜电位。

结论

P. linteus 多糖提取物诱导 THP-1 单核细胞凋亡，而 A. bisporus 和 A. brasiliensis 多糖提取物则诱导这些细胞分化为巨噬细胞。人血清的保护作用表明，P. linteus 多糖提取物通过与 TRAIL 受体结合，通过外在途径诱导细胞凋亡。线粒体膜电位和活性氧似乎在细胞分化和细胞死亡中起着重要作用。