Department of Ophthalmology, School of Medicine, Universidad Nacional de Colombia, Bogota, Colombia.
J Cataract Refract Surg. 2010 Apr;36(4):659-64. doi: 10.1016/j.jcrs.2009.11.003.
To evaluate the effect of genipin, a natural crosslinker, on porcine corneas.
Department of Ophthalmology, Universidad Nacional de Colombia, Bogota, Colombia.
Corneal strips (12.0 mm x 2.3 mm) were harvested from porcine eyes and treated by incubation with genipin at concentrations of 1.00%, 0.25%, and 0.10%. Parallel corneal strips from the same eye were used as untreated controls. After treatment at 20 degrees C for 40 minutes, tensile strain measurements were performed in a biomaterial tester. Porcine button corneas were treated with genipin 0.25% for 15 minutes and then digested by bacterial collagenase. Treated and untreated corneas were evaluated by light microscopy.
Young modulus and stiffness in treated corneas increased in a concentration-dependent manner. Genipin increased resistance to corneal collagenase 5-fold in comparison with the controls. A decrease in the interlamellar space in treated corneas was also observed.
Corneal collagen crosslinking induced with genipin produced a significant increase in biomechanical strength and resistance to bacterial collagenase. This crosslinker could be useful in treating corneal ectasia and corneal infectious and noninfectious diseases involving corneal melting.
评估天然交联剂京尼平对猪眼角膜的作用。
哥伦比亚波哥大，哥伦比亚国立大学眼科系。
从猪眼采集角膜条（12.0mm x 2.3mm），并用浓度为 1.00%、0.25%和 0.10%的京尼平孵育处理。来自同一眼睛的平行角膜条用作未处理的对照。在 20℃下处理 40 分钟后，在生物材料试验机上进行拉伸应变测量。将猪纽扣角膜用 0.25%的京尼平处理 15 分钟，然后用细菌胶原酶消化。用光学显微镜评估处理和未处理的角膜。
处理过的角膜的杨氏模量和硬度呈浓度依赖性增加。与对照组相比，京尼平将角膜胶原酶的抵抗力提高了 5 倍。还观察到处理过的角膜层间空间减少。
用京尼平诱导的角膜胶原交联可显著提高生物力学强度和对细菌胶原酶的抵抗力。这种交联剂可用于治疗涉及角膜融解的角膜扩张症和角膜感染及非传染性疾病。
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