Zhongshan Ophthalmic Center at Sun Yat-sen University, The State Key Laboratory of Ophthalmology, Guangzhou, China.
Acta Ophthalmol. 2013 Jun;91(4):e253-7. doi: 10.1111/aos.12172. Epub 2013 May 28.
To evaluate the effect of collagen cross-linking induced by genipin in porcine sclera.
Porcine cadaver eyes were treated with genipin at concentrations (by w/v) of 0.01%, 0.03%, 0.1%, 0.3%, 1.0% for 15 and 30 min. Riboflavin/ultraviolet A(UVA)-treated and untreated samples were used as controls. After treatment, scleral strips of 4.0 × 10.0 mm were cut. Twenty-four hours later, the stress-strain parameters of the strips were measured using a biomaterial microtester. The stress and Young's modulus at 8% strain were evaluated.
Compared with untreated groups, after treatment with genipin for 15 min, the stress was increased by 66-246%, depending on the concentration of genipin. As for the 30-min groups, the stress was 171-444% higher than that of the control. The difference of the Young's modulus between genipin 15-min groups, except the 0.01% groups (p = 0.095), also had statistical significance (p < 0.05). The Young's modulus had significant difference between the untreated group and the genipin 30-min groups (all p < 0.05). Of 0.3% genipin for 15 min or 0.01% genipin for 30 min had a similar stress-strain curve with those of eyes treated with the riboflavin/UVA group. The sclera exhibited a bluish colour which became deeper with increase concentration and cross-linking time.
Collagen cross-linking induced by genipin could increase the biomechanical strength in porcine sclera. The effect depends on the concentration and treatment time of genipin.
评估京尼平诱导猪巩膜胶原交联的效果。
将猪尸体眼球用浓度（w/v）为 0.01%、0.03%、0.1%、0.3%和 1.0%的京尼平处理 15 和 30 分钟。用核黄素/紫外线 A（UVA）处理和未处理的样本作为对照。处理后，切取 4.0×10.0mm 的巩膜条。24 小时后，使用生物材料微测仪测量条带的应力-应变参数。评估应变 8%时的应力和杨氏模量。
与未处理组相比，京尼平处理 15 分钟后，根据京尼平浓度的不同，应力增加了 66%-246%。对于 30 分钟组，应力比对照组高 171%-444%。除 0.01%组（p=0.095）外，京尼平 15 分钟组的杨氏模量差异也具有统计学意义（p<0.05）。未处理组与京尼平 30 分钟组的杨氏模量有显著差异（均 p<0.05）。0.3%京尼平处理 15 分钟或 0.01%京尼平处理 30 分钟的巩膜的应力-应变曲线与用核黄素/UVA 处理的眼睛相似。巩膜呈现出蓝色，随着浓度和交联时间的增加而加深。
京尼平诱导的胶原交联可以增加猪巩膜的生物力学强度。这种效果取决于京尼平的浓度和处理时间。
