Rinaldo C R, Richter B S, Black P H, Callery R, Chess L, Hirsch M S
J Immunol. 1978 Jan;120(1):130-6.
Human peripheral blood leukocytes, lymphocyte subpopulations, and hemic cell lines were examined for their ability to supprot HSV and CMV replication. Mitogen-stimulated mononuclear leukocytes, B lymphocytes, and T lymphcytes supported the replication of HSV to high titers over 3 to 5 days of infection. HSV replicated in unstimulated mononuclear leukocyte cultures of one of five donors, and to a limited degree in untreated B lymphocytes of three of five donors; HSV replication was not detected in unstimulated T lymphocytes (five donors). There was no evidence of enhanced uptake of 3H-thymidine in the untreated donor cells that replicated HSV. CMV replication was not detected during 9 to 10 days of infection in untreated or mitogen-treated mononuclear leukocytes and lymphocyte subpopulations from the same adult donors or in neonatal cord blood leukocytes. The ability of the cells to support HSV or CMV replication did not correlate with the presence of specific antiviral antibodies in the donor serum. HSV replication in B, T, and myeloid cell lines to high titers over 5 days of infection, whereas CMV failed to replicate in any of the hemic cell lines. A persistent HSV infection has been established in a T cell line (CEM) with high titers of infectious virus being produced concurrently with growth of the cells over the first 11 weeks of infection.
对人外周血白细胞、淋巴细胞亚群和血细胞系进行检测，以评估它们支持单纯疱疹病毒（HSV）和巨细胞病毒（CMV）复制的能力。在感染的3至5天内，经丝裂原刺激的单核白细胞、B淋巴细胞和T淋巴细胞能支持HSV复制至高滴度。HSV在5名供体中1名的未刺激单核白细胞培养物中复制，在5名供体中3名的未处理B淋巴细胞中有限程度地复制；在未刺激的T淋巴细胞（5名供体）中未检测到HSV复制。在复制HSV的未处理供体细胞中，没有证据表明3H-胸腺嘧啶核苷摄取增加。在来自相同成年供体的未处理或经丝裂原处理的单核白细胞和淋巴细胞亚群中，以及在新生儿脐带血白细胞中，感染9至10天期间未检测到CMV复制。细胞支持HSV或CMV复制的能力与供体血清中特异性抗病毒抗体的存在无关。在感染5天内，HSV在B、T和髓系细胞系中复制至高滴度，而CMV在任何血细胞系中均未复制。在一个T细胞系（CEM）中建立了持续性HSV感染，在感染的前11周，随着细胞生长同时产生高滴度的感染性病毒。
