基于金纳米棒和苏木素染色癌细胞的双模光学投影断层成像显微镜。

Dual-mode optical projection tomography microscope using gold nanorods and hematoxylin-stained cancer cells.

机构信息

Department of Bioengineering, University of Washington, NE Pacific St., Seattle, Washington 98195, USA.

出版信息

Opt Lett. 2010 Apr 1;35(7):1037-9. doi: 10.1364/OL.35.001037.

DOI:10.1364/OL.35.001037

PMID:20364209

Abstract

An optical projection tomography microscope (OPTM) can improve axial resolution by viewing a sample from different perspectives. Here, we report a dual-mode OPTM that can generate 3D images of single cancer cells in both absorption mode and polarization mode. Cancer cells were labeled with hematoxylin for absorption imaging and nanorods for polarization imaging. Absorption images can provide morphologic information, and polarization images can provide molecular information. The combination of molecular detection and 3D cytological cell analysis may help with early cancer diagnosis.

摘要

光学投影断层显微镜（OPTM）可以通过从不同角度观察样本来提高轴向分辨率。在这里，我们报告了一种双模 OPTM，它可以在吸收模式和偏振模式下生成单个癌细胞的 3D 图像。癌细胞用苏木精标记进行吸收成像，用纳米棒标记进行偏振成像。吸收图像可以提供形态学信息，而偏振图像可以提供分子信息。分子检测与 3D 细胞学细胞分析的结合可能有助于早期癌症诊断。