Department of Bioengineering, University of Washington, Seattle, WA 98195, USA.
Ann Biomed Eng. 2012 Feb;40(2):263-76. doi: 10.1007/s10439-011-0411-5. Epub 2011 Oct 8.
Absorption dyes are widely used in traditional cytology and pathology clinical practice, while fluorophores and nanoparticles are more often used in biologic research. Optical projection tomographic microscopy (OPTM) is a platform technology that can image the same specimen in multiple modes in 3D, providing morphologic and molecular information concurrently and in exact co-registration. The depth-of-field of a high numerical aperture objective is extended by scanning the focal plane through the sample to generate an optical projection image. Samples of cells or tissue are brought into the OPTM instrument through a microcapillary tube filled with optical index-matching gel. Multiple optical projection images are taken from different perspectives by rotating the tube. Computed tomography (CT) algorithms are applied to these optical projection images to reconstruct 3D structure of the sample. Image segmentation and analysis based on these 3D images provide quantitative biosignatures for cancer diagnosis that represents a clear improvement over conventional 2D image analysis. In this article, we introduce the OPTM platform, optical Cell-CT, and Tissue-CT instruments, and some applications using these OPTM instruments.
吸收染料广泛应用于传统细胞学和病理学的临床实践，而荧光染料和纳米粒子则更多地用于生物研究。光学投影层析显微镜（OPTM）是一种平台技术，可在 3D 中以多种模式对同一标本进行成像，同时提供形态学和分子信息，并进行精确配准。通过在样品中扫描焦平面来扩展高数值孔径物镜的景深，以生成光学投影图像。将充满光学折射率匹配凝胶的微毛细管管中的细胞或组织样本引入 OPTM 仪器中。通过旋转管从不同角度拍摄多个光学投影图像。将 CT 算法应用于这些光学投影图像中，以重建样本的 3D 结构。基于这些 3D 图像的图像分割和分析为癌症诊断提供了定量生物标志物，这明显优于传统的 2D 图像分析。在本文中，我们介绍了 OPTM 平台、光学细胞 CT 和组织 CT 仪器，以及使用这些 OPTM 仪器的一些应用。
