Pei Xiaoyan, Liu Xiumei
Institute of Nutrition and Food Safety, Chinese Center for Disease Control and Prevention, Beijing 100050, China.
Wei Sheng Yan Jiu. 2010 Jan;39(1):36-9.
To analyse the sequence of 16S rDNA of twenty-nine Cronobacter spp. strains isolated from food as well as two type strains.
Using polymerase chain reaction to obtain the sequences of 16S rDNA of all strains. The multiple cluster analysis and phylogenetic analysis of them were studied by BioNumerics software.
All strains, except ATCC51329 and ES 014, were clustered with the Cronobacter spp. type strain ATCC29544. The Cronobacter spp. type strain ATCC51329 and ES 014 belonged to a different branch. From the sequence analysis of 16S rDNA, all these isolates of Cronobacter spp. except ES 014 could be identified accurately by gene analysis.
All Cronobacter spp. strains isolated from food were identified on genetic level. The phylogenetic relationships among related Cronobacter spp. isolates were studied.
分析从食品中分离出的29株阪崎肠杆菌属菌株以及两株模式菌株的16S rDNA序列。
采用聚合酶链反应获取所有菌株的16S rDNA序列。利用BioNumerics软件对其进行多重聚类分析和系统发育分析。
除ATCC51329和ES 014外，所有菌株均与阪崎肠杆菌属模式菌株ATCC29544聚类。阪崎肠杆菌属模式菌株ATCC51329和ES 014属于不同分支。通过16S rDNA序列分析，除ES 014外，所有这些阪崎肠杆菌属分离株均可通过基因分析准确鉴定。
从食品中分离出的所有阪崎肠杆菌属菌株均在基因水平上得到鉴定。对相关阪崎肠杆菌属分离株之间的系统发育关系进行了研究。
