Del Maestro R F, Farrell C R, Stroude E, McDonald W
Department of Clinical Neurological Sciences, University of Western Ontario, Victoria Hospital, London, Canada.
Can J Neurol Sci. 1991 Feb;18(1):7-11. doi: 10.1017/s0317167100031231.
A potent in vitro inhibitor of lipid peroxidation, U78517F, has been employed to assess its influence on C6 astrocytoma growth in monolayer culture and tumor growth and protein extravasation in the rat C6 astrocytoma spheroid implantation model. Results demonstrate that 1 microM concentration of U78517F inhibits cell division, but is not tumoricidal to C6 astrocytoma cells in monolayer culture. Concentrations of 5 microM and above significantly decreased astrocytoma cell viability. Following spheroid implantation, rats were treated with one of these U78517F regimes: 12 mg/kg/day for 13 days post-implantation, 4 mg/kg/day for 13 days post-implantation or 12 mg/kg/day commencing seven days post-implantation until 13 days post-implantation. Tumor wet and dry weights were lower in all treatment groups, but these decreases were not statistically significant. Protein extravasation as measured by Evans blue extravasation was not significantly reduced by any treatment regime used. It is concluded that U78517F inhibits C6 astrocytoma growth in monolayer culture at and above 5 microM concentrations, but the treatment regimes utilized did not significantly decrease tumor growth or permeability in the C6 astrocytoma spheroid implantation model.
一种强效的体外脂质过氧化抑制剂U78517F已被用于评估其对单层培养的C6星形细胞瘤生长以及大鼠C6星形细胞瘤球体植入模型中肿瘤生长和蛋白质外渗的影响。结果表明，1 microM浓度的U78517F可抑制细胞分裂，但对单层培养的C6星形细胞瘤细胞无杀瘤作用。5 microM及以上浓度可显著降低星形细胞瘤细胞活力。在球体植入后，大鼠接受以下U78517F治疗方案之一：植入后13天内每天12 mg/kg，植入后13天内每天4 mg/kg，或植入后7天开始至植入后13天每天12 mg/kg。所有治疗组的肿瘤湿重和干重均较低，但这些降低无统计学意义。通过伊文思蓝外渗测量的蛋白质外渗未因任何使用的治疗方案而显著降低。结论是，U78517F在5 microM及以上浓度时可抑制单层培养的C6星形细胞瘤生长，但所采用的治疗方案并未显著降低C6星形细胞瘤球体植入模型中的肿瘤生长或通透性。
