National Centre for Cell Science, NCCS Complex, Pune University Campus, Ganeshkhind, Pune 411 007, India.
Reproduction. 2010 Jul;140(1):33-41. doi: 10.1530/REP-09-0389. Epub 2010 Mar 16.
The isolation and identification of stem-like cells in solid tumors or cancer stem cells (CSCs) have been exciting developments of the last decade, although these rare populations had been earlier identified in leukemia. CSC biology necessitates a detailed delineation of normal stem cell functioning and maintenance of homeostasis within the organ. Ovarian CSC biology has unfortunately not benefited from a pre-established knowledge of stem cell lineage demarcation and functioning in the normal organ. In the absence of such information, some of the classical parameters such as long-term culture-initiating assays to isolate stem cell clones from tumors, screening and evaluation of other epithelial stem cell surface markers, dye efflux, and label retention have been applied toward the putative isolation of CSCs from ovarian tumors. The present review presents an outline of the various approaches developed so far and the various perspectives revealed that are now required to be dealt with toward better disease management.
过去十年中，实体肿瘤或癌症干细胞（CSC）中的干样细胞的分离和鉴定是令人兴奋的进展，尽管这些稀有群体在白血病中更早被发现。CSC 生物学需要详细描述正常干细胞的功能和器官内的内稳态维持。不幸的是，卵巢 CSC 生物学没有从正常器官中干细胞谱系划分和功能的既定知识中受益。在缺乏此类信息的情况下，一些经典参数，如从肿瘤中分离干细胞克隆的长期培养起始测定、筛选和评估其他上皮干细胞表面标志物、染料外排和标记保留，已被应用于从卵巢肿瘤中分离 CSC。本文综述了迄今为止开发的各种方法，并揭示了现在需要处理的各种观点，以更好地进行疾病管理。
