发现大肠杆菌中一种赋予多种氨基糖苷类药物耐药性的基因。
Discovery of a gene conferring multiple-aminoglycoside resistance in Escherichia coli.
机构信息
Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA 99164-7040, USA.
出版信息
Antimicrob Agents Chemother. 2010 Jun;54(6):2666-9. doi: 10.1128/AAC.01743-09. Epub 2010 Apr 5.
Abstract
Bovine-origin Escherichia coli isolates were tested for resistance phenotypes using a disk diffusion assay and for resistance genotypes using a DNA microarray. An isolate with gentamicin and amikacin resistance but with no corresponding genes detected yielded a 1,056-bp DNA sequence with the closest homologues for its inferred protein sequence among a family of 16S rRNA methyltransferase enzymes. These enzymes confer high-level aminoglycoside resistance and have only recently been described in Gram-negative bacteria.
摘要
使用纸片扩散法检测牛源大肠杆菌分离株的耐药表型，使用 DNA 微阵列检测耐药基因型。一个对庆大霉素和阿米卡星有耐药性但未检测到相应基因的分离株产生了一个 1056 碱基对的 DNA 序列，其推测蛋白序列与一组 16S rRNA 甲基转移酶酶的最接近同源物。这些酶赋予了高水平的氨基糖苷类抗生素耐药性，并且最近才在革兰氏阴性菌中被描述。
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