Xu Chen-Xiang, Mao Jun-Biao, Jiang Rui
Department of Urology, Affiliated Hospital of Luzhou Medical College, Luzhou, Sichuan 646000, China.
Zhonghua Nan Ke Xue. 2010 Feb;16(2):112-7.
To study the expressions of Erk1/2 and PKB/Akt in the corpus cavernosum of castrated rats and investigate their action mechanism in the development of erectile dysfunction (ED) after castration.
We randomly divided 20 eight-week-old SD rats into Groups A (sham-operation) and B (castration), and, 4 weeks after the operation, determined the level of serum testosterone (T) and the expressions of P-Erk1/2 and P-PKB/Akt proteins (integrated optical density/area, IA/area) and those of Erk1/2 and PKB/Akt mRNA (Marker: GAPDH) in the corpus cavernosum of the rats by immunohistochemical staining and RT-PCR.
Four weeks after the operation, the serum T level was significantly decreased in Group B in comparison with A ([10.090 +/- 3. 026] nmol/L versus [1.339 +/- 0.642] nmol/L, P < 0.05). Erk1/2 and PKB/Akt expressed in the corpus cavernosum of both groups of rats. The expressions of Erk1 and Erk2 mRNA and P-Erk1/2 were significantly higher in Group B (0. 840 +/- 0.062, 0.876 +/- 0.141 and 0.142 +/- 0.020) than in A (0.479 +/- 0.090, 0.599 +/- 0.100 and 0.119 +/- 0.029) (P < 0.05). But no statistically significant differences were found in the expressions of PKB/Akt mRNA and P-PKB/Akt between Groups B (0.974 +/- 0.040 and 0.164 +/- 0.036) and A (0.942 +/- 0.054 and 0.162 +/- 0.025) (P < 0.05).
Erk1/2 and PKB/Akt expressed in the penile tissues of both castrated and sham-operation rats. The increased expression of P-Erk1/2 in the corpus cavernosum may be involved in the development of ED in castrated rats.
研究去势大鼠阴茎海绵体中细胞外信号调节激酶1/2（Erk1/2）和蛋白激酶B/蛋白激酶B（PKB/Akt）的表达情况，并探讨它们在去势后勃起功能障碍（ED）发生发展中的作用机制。
将20只8周龄的SD大鼠随机分为A组（假手术组）和B组（去势组），术后4周，通过免疫组织化学染色和逆转录-聚合酶链反应（RT-PCR）检测大鼠血清睾酮（T）水平、阴茎海绵体中磷酸化Erk1/2（P-Erk1/2）和磷酸化PKB/Akt蛋白的表达水平（积分光密度/面积，IA/面积）以及Erk1/2和PKB/Akt信使核糖核酸（mRNA）的表达水平（内参：甘油醛-3-磷酸脱氢酶，GAPDH）。
术后4周，B组血清T水平较A组显著降低（[10.090±3.026]纳摩尔/升对[1.339±0.642]纳摩尔/升，P<0.05）。两组大鼠阴茎海绵体中均有Erk1/2和PKB/Akt表达。B组Erk1和Erk2 mRNA及P-Erk1/2的表达水平（0.840±0.062、0.876±0.141和0.142±0.020）显著高于A组（0.479±0.090、0.599±0.100和0.119±0.029）（P<0.05）。但B组（0.974±0.040和0.164±0.036）与A组（0.942±0.054和0.162±0.025）之间PKB/Akt mRNA及P-PKB/Akt的表达差异无统计学意义（P>0.05）。
去势大鼠和假手术大鼠阴茎组织中均有Erk1/2和PKB/Akt表达。阴茎海绵体中P-Erk1/2表达增加可能参与了去势大鼠ED的发生发展。
