[胱硫醚-β-合酶和胱硫醚-γ-裂解酶在去势大鼠阴茎海绵体平滑肌中的表达]

[Expressions of cystathionine-beta-synthase and cystathionine-gamma-lyase in the corpus cavernosum smooth muscle of castrated rats].

作者信息

Luo Hua, Liu Lun-Bo, Feng Ai-Ping, Gao Yang, Liao Gao-Yuan, Qiu Ming-Quan

机构信息

Department of Urology, The Second People's Hospital of Deyang City, Deyang, Sichuan 618000, China.

出版信息

Zhonghua Nan Ke Xue. 2013 Jul;19(7):599-603.

PMID:23926675

Abstract

OBJECTIVE

To study the expressions of cystathionine-beta-synthase (CBS) and cystathionine-gamma-lyase (CSE) in the corpus cavernosum smooth muscle of castrated rats and their roles in erectile dysfunction after castration.

METHODS

We randomly assigned 40 eight-week-old male SD rats to groups A (2-week sham-operation), B (4-week sham-operation), C (2-week castration) and D (4-week castration). We determined the level of serum testosterone (T) and the expressions of CBS and CSE in the corpus cavernosum smooth muscle of the rats after operation using immunohistochemistry and RT-PCR.

RESULTS

The T level was significantly decreased in groups C ([11.85 +/- 6.73] nmol/L) and D ([1.96 +/- 1.23] nmol/L) as compared with A ([89.65 +/- 17.13] nmol/L) and B ([106.75 +/- 19.68] nmol/L) (P < 0.05). CBS and CSE were expressed in all groups of rats, but the relative expressions of CBS and CSE mRNA were significantly lower in groups C (0.93 +/- 0.14 and 0.87 +/- 0.20) and D (0.79 +/- 0.17 and 0.71 +/- 0.12) than in A (2.13 +/- 0.65 and 1.93 +/- 0.15) and B (2.07 +/- 0.53 and 1.89 +/- 0.45) (P < 0. 05), so were the optical density values (IA) of the CBS and CSE proteins, 130.35 +/- 23.56 and 93.56 +/- 36.64 in group C and 80.29 +/- 29.65 and 58.56 +/- 19.95 in group D, as compared with 310.57 +/- 130.56 and 269.56 +/- 116.76 in group A and 349.68 +/-112.35 and 298.35 +/- 100.76 in group B (P < 0.05). The androgen level was positively correlated with the expressions of CBS and CSE in the corpus cavernosum smooth muscle of the rats.

CONCLUSION

Androgen regulates erectile function via the expressions of CBS and CSE.

摘要

目的

研究胱硫醚-β-合酶（CBS）和胱硫醚-γ-裂解酶（CSE）在去势大鼠阴茎海绵体平滑肌中的表达及其在去势后勃起功能障碍中的作用。

方法

将40只8周龄雄性SD大鼠随机分为A组（假手术2周）、B组（假手术4周）、C组（去势2周）和D组（去势4周）。术后采用免疫组织化学和RT-PCR法检测大鼠血清睾酮（T）水平及阴茎海绵体平滑肌中CBS和CSE的表达。

结果

与A组（[89.65±17.13]nmol/L）和B组（[106.75±19.68]nmol/L）相比，C组（[11.85±6.73]nmol/L）和D组（[1.96±1.23]nmol/L）的T水平显著降低（P<0.05）。所有组大鼠均表达CBS和CSE，但C组（0.93±0.14和0.87±0.20）和D组（0.79±0.17和0.71±0.12）的CBS和CSE mRNA相对表达量显著低于A组（2.13±0.65和1.93±0.15）和B组（2.07±0.53和1.89±0.45）（P<0.05），CBS和CSE蛋白的光密度值（IA）也如此，C组为130.35±23.56和93.56±36.64，D组为80.29±29.65和58.56±19.95，而A组为310.57±130.56和269.56±116.76，B组为349.68±112.35和298.35±100.76（P<0.05）。雄激素水平与大鼠阴茎海绵体平滑肌中CBS和CSE的表达呈正相关。