Hiriart M, Ramirez-Medeles M C
Department of Neurosciences, Universidad Nacional Autonoma de Mexico, Mexico City.
Endocrinology. 1991 Jun;128(6):3193-8. doi: 10.1210/endo-128-6-3193.
In the present study we have used a reverse hemolytic plaque assay and frequency distribution analysis to characterize the insulin secretory response of individual rat pancreatic B-cells in culture. Heterogeneous secretory behavior was observed with a multimodal distribution, suggesting the existence of B-cell subpopulations. Insulin secretion in response to increased extracellular glucose involved both amplification of secretion by individual cells as well as recruitment of previously silent B-cells.
在本研究中，我们使用了反向溶血空斑测定法和频率分布分析来表征培养的单个大鼠胰腺β细胞的胰岛素分泌反应。观察到具有多峰分布的异质分泌行为，提示存在β细胞亚群。对细胞外葡萄糖增加的胰岛素分泌反应既涉及单个细胞分泌的放大，也涉及先前沉默的β细胞的募集。
