Plumas J, Gruart V, Aldebert D, Truong M J, Capron M, Capron A, Prin L
Centre d'Immunologie et de Biologie Parasitaire, Unité Mixte INSERM U 167-CNRS 624, Institut Pasteur, Lille, France.
Eur J Immunol. 1991 May;21(5):1265-70. doi: 10.1002/eji.1830210525.
The expression of interleukin 2 receptor (IL2R) on eosinophils was investigated in patients with hypereosinophilia. Hypodense activated eosinophils have been described in various diseases such as parasitic or allergic diseases, hypereosinophilic syndrome (HES) associated in some cases to myeloproliferative markers, and more recently described in patients undergoing recombinant IL2 treatment. The presence of p55 alpha chain of IL2R (CD25) on purified eosinophils collected from blood of hypereosinophilic patients was detected by flow cytometry. In 10 out of 19 cases, more than 10% of eosinophils were CD25+. Cross-linking studies on enriched eosinophils showed one 64-75-kDa band, consisting of IL2 (15 kDa) cross-linked to the IL2R p55 subunit. In Northern blot analysis the two messenger mRNA (3.5 and 1.5 kb) encoding the IL2R p55 subunit were identified after hybridization with a CD25 cDNA probe. In contrast, the presence of the IL2R p75 subunit was not detected. These data provide preliminary evidence for the expression of a low-affinity receptor for IL2 on in vivo activated eosinophils and raise the question of the role played by this cytokine in eosinophil differentiation and activation.
在嗜酸性粒细胞增多症患者中研究了嗜酸性粒细胞上白细胞介素2受体（IL2R）的表达。低密度活化嗜酸性粒细胞已在多种疾病中被描述，如寄生虫病或过敏性疾病、某些情况下与骨髓增殖标志物相关的嗜酸性粒细胞增多综合征（HES），以及最近在接受重组IL2治疗的患者中被描述。通过流式细胞术检测从嗜酸性粒细胞增多症患者血液中收集的纯化嗜酸性粒细胞上IL2R的p55α链（CD25）的存在情况。在19例中的10例中，超过10%的嗜酸性粒细胞为CD25阳性。对富集的嗜酸性粒细胞进行的交联研究显示一条64 - 75 kDa的条带，由与IL2R p55亚基交联的IL2（15 kDa）组成。在Northern印迹分析中，用CD25 cDNA探针杂交后鉴定出编码IL2R p55亚基的两种信使mRNA（3.5和1.5 kb）。相反，未检测到IL2R p75亚基的存在。这些数据为体内活化的嗜酸性粒细胞上IL2低亲和力受体的表达提供了初步证据，并提出了这种细胞因子在嗜酸性粒细胞分化和活化中所起作用的问题。
